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生物打印:再生医学中的机械稳定与强化策略

Bioprinting: Mechanical Stabilization and Reinforcement Strategies in Regenerative Medicine.

作者信息

Ballard Ashleigh, Patush Rebecca, Perez Jenesis, Juarez Carmen, Kirillova Alina

机构信息

Department of Materials Science and Engineering, Iowa State University, Ames, Iowa, USA.

Des Moines Area Community College, Ankeny, Iowa, USA.

出版信息

Tissue Eng Part A. 2024 Jul;30(13-14):387-408. doi: 10.1089/ten.TEA.2023.0239. Epub 2024 Feb 9.

Abstract

Bioprinting describes the printing of biomaterials and cell-laden or cell-free hydrogels with various combinations of embedded bioactive molecules. It encompasses the precise patterning of biomaterials and cells to create scaffolds for different biomedical needs. There are many requirements that bioprinting scaffolds face, and it is ultimately the interplay between the scaffold's structure, properties, processing, and performance that will lead to its successful translation. Among the essential properties that the scaffolds must possess-adequate and appropriate application-specific chemical, mechanical, and biological performance-the mechanical behavior of hydrogel-based bioprinted scaffolds is the key to their stable performance at the site of implantation. Hydrogels that typically constitute the main scaffold material and the medium for the cells and biomolecules are very soft, and often lack sufficient mechanical stability, which reduces their printability and, therefore, the bioprinting potential. The aim of this review article is to highlight the reinforcement strategies that are used in different bioprinting approaches to achieve enhanced mechanical stability of the bioinks and the printed scaffolds. Enabling stable and robust materials for the bioprinting processes will lead to the creation of truly complex and remarkable printed structures that could accelerate the application of smart, functional scaffolds in biomedical settings. Impact statement Bioprinting is a powerful tool for the fabrication of 3D structures and scaffolds for biomedical applications. It has gained tremendous attention in recent years, and the bioink library is expanding to include more and more material combinations. From the practical application perspective, different properties need to be considered, such as the printed structure's chemical, mechanical, and biological performances. Among these, the mechanical behavior of the printed constructs is critical for their successful translation into the clinic. The aim of this review article is to explore the different reinforcement strategies used for the mechanical stabilization of bioinks and bioprinted structures.

摘要

生物打印是指打印生物材料以及含有细胞或不含细胞的水凝胶,并嵌入各种组合的生物活性分子。它包括对生物材料和细胞进行精确图案化,以创建满足不同生物医学需求的支架。生物打印支架面临许多要求,最终是支架的结构、特性、加工和性能之间的相互作用将导致其成功转化应用。在支架必须具备的基本特性中,即足够且合适的特定应用化学、机械和生物学性能,基于水凝胶的生物打印支架的力学行为是其在植入部位稳定性能的关键。通常构成主要支架材料以及细胞和生物分子介质的水凝胶非常柔软,往往缺乏足够的机械稳定性,这降低了它们的可打印性,进而影响生物打印潜力。本文综述的目的是强调在不同生物打印方法中用于增强生物墨水和打印支架机械稳定性的增强策略。使生物打印过程能够使用稳定且坚固的材料将有助于创建真正复杂且卓越的打印结构,从而加速智能、功能性支架在生物医学领域的应用。影响声明生物打印是用于制造生物医学应用的三维结构和支架的强大工具。近年来它受到了极大关注,生物墨水库也在不断扩大,纳入了越来越多的材料组合。从实际应用角度来看,需要考虑不同的特性,例如打印结构的化学、机械和生物学性能。其中,打印构建体的力学行为对于其成功转化应用于临床至关重要。本文综述的目的是探索用于生物墨水和生物打印结构机械稳定化的不同增强策略。

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