Tang Ling-Tong, Feng Lei, Cao Hui-Ying, Shi Rui, Luo Bei-Bei, Zhang Yan-Bi, Liu Yan-Mei, Zhang Jian, Li Shuang-Yue
Department of Clinical Laboratory, Yan'an Hospital Affiliated to Kunming Medical University, Kunming 650051, Yunnan Province, China.
Department of Clinical Laboratory, Sixth Affiliated Hospital of Kunming Medical University, Kunming 650051, Yunnan Province, China.
World J Diabetes. 2023 Dec 15;14(12):1766-1783. doi: 10.4239/wjd.v14.i12.1766.
The global prevalence of type 2 diabetes mellitus (T2DM) is increasing. T2DM is associated with alterations of the gut microbiota, which can be affected by age, illness, and genetics. Previous studies revealed that there are discriminating microbiota compositions between the Dai and the Han populations. However, the specific gut microbiota differences between the two populations have not been elucidated.
To compare the gut microbiota differences in subjects with and without T2DM in the Dai and Han populations.
A total of 35 subjects of the Han population (including 15 healthy children, 8 adult healthy controls, and 12 adult T2DM patients) and 32 subjects of the Dai population (including 10 healthy children, 10 adult healthy controls, and 12 adult T2DM patients) were enrolled in this study. Fasting venous blood samples were collected from all the subjects for biochemical analysis. Fecal samples were collected from all the subjects for DNA extraction and 16S rRNA sequencing, which was followed by analyses of the gut microbiota composition.
No significant difference in alpha diversity was observed between healthy children and adults. The diversity of gut microbiota was decreased in T2DM patients compared to the healthy adults in both the Dai and Han populations. There was a significant difference in gut microbiota between healthy children and healthy adults in the Han population with an increased abundance of Bacteroidetes and decreased Firmicutes in children. However, this difference was less in the Dai population. Significant increases in Bacteroidetes in the Han population and Proteobacteria in the Dai population and decreases in Firmicutes in both the Han and Dai population were observed in T2DM patients compared to healthy adults. Linear discriminant analysis Effect Size analysis also showed that the gut microbiota was different between the Han and Dai populations in heathy children, adults, and T2DM patients. Four bacteria were consistently increased and two consistently decreased in the Han population compared to the Dai population.
Differences in gut microbiota were found between the Han and Dai populations. A significant increase in Bacteroidetes was related to the occurrence of T2DM in the Han population, while a significant increase in Proteobacteria was related to the occurrence of T2DM in the Dai population.
2型糖尿病（T2DM）的全球患病率正在上升。T2DM与肠道微生物群的改变有关，而肠道微生物群会受到年龄、疾病和遗传因素的影响。先前的研究表明，傣族和汉族人群的微生物群组成存在差异。然而，这两个人群之间具体的肠道微生物群差异尚未阐明。
比较傣族和汉族人群中患T2DM和未患T2DM的受试者的肠道微生物群差异。
本研究共纳入35名汉族受试者（包括15名健康儿童、8名成年健康对照者和12名成年T2DM患者）和32名傣族受试者（包括10名健康儿童、10名成年健康对照者和12名成年T2DM患者）。采集所有受试者的空腹静脉血样本进行生化分析。采集所有受试者的粪便样本进行DNA提取和16S rRNA测序，随后分析肠道微生物群组成。
健康儿童和成年人之间未观察到α多样性的显著差异。与傣族和汉族人群中的健康成年人相比，T2DM患者的肠道微生物群多样性降低。汉族人群中健康儿童和健康成年人的肠道微生物群存在显著差异，儿童中拟杆菌门丰度增加，厚壁菌门丰度降低。然而，傣族人群中的这种差异较小。与健康成年人相比，T2DM患者中汉族人群的拟杆菌门显著增加，傣族人群的变形菌门显著增加，汉族和傣族人群的厚壁菌门均减少。线性判别分析效应大小分析还表明，健康儿童、成年人和T2DM患者中，汉族和傣族人群的肠道微生物群不同。与傣族人群相比，汉族人群中有4种细菌持续增加，2种细菌持续减少。
发现汉族和傣族人群的肠道微生物群存在差异。汉族人群中拟杆菌门的显著增加与T2DM的发生有关，而傣族人群中变形菌门的显著增加与T2DM的发生有关。
