Pfitzinger H, Guillemaut P, Weil J H, Pillay D T
Nucleic Acids Res. 1987 Feb 25;15(4):1377-86. doi: 10.1093/nar/15.4.1377.
In chloroplasts there is a correlation between the amounts of tRNAs specific for a given amino acid and the codons specifying this amino acid. Furthermore, for the amino acids coded for by more than one codon, the population of isoaccepting tRNAs is adjusted to the frequency of synonymous codons used in chloroplast protein genes. A comparison by two-dimensional gel electrophoresis of the tRNA populations extracted from chloroplasts and from chloroplast polysomes shows that all chloroplast tRNAs are involved in protein biosynthesis.
在叶绿体中,特定氨基酸的tRNA数量与编码该氨基酸的密码子之间存在相关性。此外,对于由多个密码子编码的氨基酸,同功tRNA群体根据叶绿体蛋白质基因中同义密码子的使用频率进行调整。通过二维凝胶电泳对从叶绿体和叶绿体多核糖体中提取的tRNA群体进行比较,结果表明所有叶绿体tRNA都参与蛋白质生物合成。