Prelipcean Ana-Maria, Iosageanu Andreea, Gaspar-Pintiliescu Alexandra, Moldovan Lucia, Craciunescu Oana, Negreanu-Pirjol Ticuta, Negreanu-Pirjol Bogdan, Mitran Raul-Augustin, Marin Mariana, D'Amora Ugo
National Institute of R&D for Biological Sciences, 296 Splaiul Independentei, 060031 Bucharest, Romania.
Faculty of Pharmacy, University "Ovidius" of Constanta, 1 Aleea Universității, 900470 Constanta, Romania.
Materials (Basel). 2022 May 13;15(10):3507. doi: 10.3390/ma15103507.
Over the past years, research attention has been focusing more on waste-derived, naturally derived, and renewable materials, in the view of a more sustainable economy. In this work, different topical formulations were obtained from the valorization of marine and agro-industrial by-products and the use of Carbopol 940 as gelling agent. In particular, the combination of extracts obtained from the marine snail, with and grape pomace extracts, was investigated for wound healing purposes. has demonstrated wound healing properties and antioxidant activity. Similarly, grape pomace extracts have been shown to accelerate the healing process. However, their synergic use has not been explored yet. To this aim, four different formulations were produced. Three formulations differed for the presence of a different extract of : marine collagen, marine gelatin, and collagen hydrolysate, while another formulation used mammalian gelatin as further control. Physico-chemical properties of the extracts as well as of the formulations were analyzed. Furthermore, thermal stability was evaluated by thermogravimetric analysis. Antioxidant capacity and biological behavior, in terms of cytocompatibility, wound healing, and antimicrobial potential, were assessed. The results highlighted for all the formulations (i) a good conservation and thermal stability in time, (ii) a neutralizing activity against free radicals, (iii) and high degree of cytocompatibility and tissue regeneration potential. In particular, collagen, gelatin, and collagen hydrolysate obtained from the marine snail represent an important, valuable alternative to mammalian products.
在过去几年中，出于更可持续经济的考虑，研究注意力更多地集中在废物衍生、天然衍生和可再生材料上。在这项工作中，通过对海洋和农业工业副产品进行增值利用以及使用卡波姆940作为胶凝剂，获得了不同的局部用制剂。特别地，研究了从海蜗牛提取物与葡萄皮渣提取物的组合用于伤口愈合的目的。海蜗牛提取物已证明具有伤口愈合特性和抗氧化活性。同样，葡萄皮渣提取物已被证明能加速愈合过程。然而，它们的协同使用尚未被探索。为此，制备了四种不同的制剂。三种制剂因存在不同的提取物而有所不同：海洋胶原蛋白、海洋明胶和胶原蛋白水解物，而另一种制剂使用哺乳动物明胶作为进一步的对照。分析了提取物以及制剂的物理化学性质。此外，通过热重分析评估了热稳定性。评估了抗氧化能力以及在细胞相容性、伤口愈合和抗菌潜力方面的生物学行为。结果突出显示了所有制剂的以下特点：（i）随时间具有良好的保存性和热稳定性，（ii）对自由基具有中和活性，（iii）高度的细胞相容性和组织再生潜力。特别地，从海蜗牛获得的胶原蛋白、明胶和胶原蛋白水解物是哺乳动物产品的重要且有价值的替代品。
