Afordoanyi Daniel Mawuena, Akosah Yaw Abayie, Shnakhova Lidiya, Saparmyradov Keremli, Diabankana Roderic Gilles Claret, Validov Shamil
Laboratory of Molecular Genetics and Microbiology Methods, Kazan Scientific Center of Russian Academy of Sciences, 420111 Kazan, Russia.
Tatar Scientific Research Institute of Agricultural Chemistry and Soil Science, FRC Kazan Scientific Center, Russian Academy of Sciences, 420111 Kazan, Russia.
Microorganisms. 2023 Dec 31;12(1):88. doi: 10.3390/microorganisms12010088.
Anthropogenic pollution, including residues from the green revolution initially aimed at addressing food security and healthcare, has paradoxically exacerbated environmental challenges. The transition towards comprehensive green biotechnology and bioremediation, achieved with lower financial investment, hinges on microbial biotechnology, with the genus emerging as a promising contender. The significance of fully annotating genome sequences lies in comprehending strain constituents, devising experimental protocols, and strategically deploying these strains to address pertinent issues using pivotal genes. This study revolves around MGMM8, an associate of winter wheat plants in the rhizosphere. Through the annotation of its chromosomal genome and subsequent comparison with other strains, its potential applications were explored. Using the antiSMASH server, 19 gene clusters were predicted, encompassing genes responsible for antibiotics and siderophores. Antibiotic resistance evaluation via the Comprehensive Antibiotic Resistance Database (CARD) identified five genes (, , , , and ) that were parallel to strain CCM2595. Leveraging the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) for biodegradation, heavy metal resistance, and remediation genes, the presence of chlorimuron-ethyl, formaldehyde, benzene-desulfurization degradation genes, and heavy metal-related genes (, , , , , and ) in MGMM8 was confirmed. Furthermore, quorum-quenching signal genes, critical for curbing biofilm formation and virulence elicited by quorum-sensing in pathogens, were also discerned within MGMM8's genome. In light of these predictions, the novel isolate MGMM8 warrants phenotypic assessment to gauge its potential in biocontrol and bioremediation. This evaluation extends to isolating active compounds for potential antimicrobial activities against pathogenic microorganisms. The comprehensive genome annotation process has facilitated the genetic characterization of MGMM8 and has solidified its potential as a biotechnological strain to address global anthropogenic predicaments.
人为污染，包括最初旨在解决粮食安全和医疗保健问题的绿色革命产生的残留物，却自相矛盾地加剧了环境挑战。向全面的绿色生物技术和生物修复的转变，以较低的资金投入实现，关键在于微生物生物技术，其中该属正成为一个有前途的竞争者。对基因组序列进行全面注释的意义在于了解菌株成分、设计实验方案，并利用关键基因战略性地部署这些菌株以解决相关问题。本研究围绕MGMM8展开，它是冬小麦根际的一种伴生菌。通过对其染色体基因组进行注释并与其他菌株进行后续比较，探索了其潜在应用。使用antiSMASH服务器，预测了19个基因簇，包括负责抗生素和铁载体的基因。通过综合抗生素抗性数据库（CARD）进行的抗生素抗性评估确定了五个与菌株CCM2595平行的基因（、、、和）。利用NCBI原核生物基因组注释管道（PGAP）来分析生物降解、重金属抗性和修复基因，证实了MGMM8中存在氯嘧磺隆、甲醛、苯脱硫降解基因以及重金属相关基因（、、、、和）。此外，在MGMM8的基因组中还发现了群体淬灭信号基因，这些基因对于抑制病原体群体感应引发的生物膜形成和毒力至关重要。鉴于这些预测，新型分离株MGMM8需要进行表型评估，以衡量其在生物防治和生物修复方面的潜力。这种评估还包括分离具有潜在抗病原微生物抗菌活性的活性化合物。全面的基因组注释过程促进了MGMM8的遗传特征分析，并巩固了其作为解决全球人为困境的生物技术菌株的潜力。
