Wang Mengjiao, Sun Haiyan, Xu Zhimin
School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China.
Collaborative Innovation Center for Comprehensive Development of Biological Resources in Qinling-Ba Mountains, Hanzhong 723000, China.
Plants (Basel). 2024 Jan 22;13(2):329. doi: 10.3390/plants13020329.
Plant rhizosphere microorganisms play an important role in modulating plant growth and productivity. This study aimed to elucidate the diversity of rhizosphere microorganisms at the flowering and fruiting stages of rapeseed (). Microbial communities in rhizosphere soils were analyzed via high-throughput sequencing of 16S rRNA for bacteria and internal transcribed spacer (ITS) DNA regions for fungi. A total of 401 species of bacteria and 49 species of fungi in the rhizosphere soil samples were found in three different samples. The composition and diversity of rhizosphere microbial communities were significantly different at different stages of rapeseed growth. Plant-growth-promoting rhizobacteria (PGPRs) have been widely applied to improve plant growth, health, and production. Thirty-four and thirty-one PGPR strains were isolated from the rhizosphere soil samples collected at the flowering and fruiting stages of rapeseed, respectively. Different inorganic phosphorus- and silicate-solubilizing and auxin-producing capabilities were found in different strains, in addition to different heavy-metal resistances. This study deepens the understanding of the microbial diversity in the rapeseed rhizosphere and provides a microbial perspective of sustainable rapeseed cultivation.
植物根际微生物在调节植物生长和生产力方面发挥着重要作用。本研究旨在阐明油菜开花和结果期根际微生物的多样性。通过对细菌的16S rRNA和真菌的内部转录间隔区（ITS）DNA区域进行高通量测序，分析根际土壤中的微生物群落。在三个不同样本中，共发现根际土壤样本中有401种细菌和49种真菌。油菜生长的不同阶段，根际微生物群落的组成和多样性存在显著差异。植物促生根际细菌（PGPRs）已被广泛应用于促进植物生长、健康和产量。分别从油菜开花期和结果期采集的根际土壤样本中分离出34株和31株PGPR菌株。除了不同的重金属抗性外，不同菌株还具有不同的无机磷和硅酸盐溶解能力以及生长素产生能力。本研究加深了对油菜根际微生物多样性的理解，并为油菜可持续种植提供了微生物视角。
