Tecnológico Nacional de México/Instituto Tecnológico Superior de Acayucan, Acayucan, Veracruz, Mexico.
Centro de Investigaciones Biológicas del Noroeste S.C, Unidad Guerrero Negro, Baja California Sur, Mexico.
Methods Mol Biol. 2024;2759:149-156. doi: 10.1007/978-1-0716-3654-1_14.
Guarianthe skinneri (Bateman) Dressler & W. E. Higgins is an orchid valued for its ornamental characteristics. However, it is an orchid classified as threatened with extinction due to the illegal extraction from its natural habitat. In addition, its propagation through seed germination is very low, as is the case with most members of the family Orchidaceae. Its asexual propagation through pseudobulb separation is slow and produces a few propagules. For this reason, in vitro propagation techniques are an alternative to increase the number of plants obtained and thus be able to recover this valuable plant genetic resource. Temporary immersion systems (TIS) offer the advantage of mass-propagating plants for different purposes. This chapter describes a large-scale micropropagation protocol for Guarianthe skinneri using temporary immersion bioreactors (TIB).
香荚兰（Bateman）Dressler 和 W. E. Higgins 是一种以观赏特性而备受重视的兰花。然而，由于非法从其自然栖息地中提取，它是一种被列为濒危物种的兰花。此外，它通过种子萌发进行繁殖的效率非常低，大多数兰科植物也是如此。通过假鳞茎分离进行无性繁殖速度较慢，产生的繁殖体较少。因此，体外繁殖技术是增加获得植物数量的一种替代方法，从而能够恢复这种有价值的植物遗传资源。临时浸泡系统（TIS）具有为不同目的大规模繁殖植物的优势。本章描述了使用临时浸泡生物反应器（TIB）对香荚兰进行大规模微繁殖的方案。
