Wang Jing, Duan Zhaojun, Luo Dixian
Department of Laboratory Medicine, Shenzhen Hospital of Integrated Traditional and Western Medicine, Shenzhen, China.
National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Beijing, China.
Research (Wash D C). 2024 Jan 29;7:0296. doi: 10.34133/research.0296. eCollection 2024.
In the post-COVID-19 pandemic era, the long-term surveillance of pathogens is still important. The rapid detection of pathogens facilitates the accurate and convenient real-time monitoring of microbial contamination and improves the management of diseases. Here, a novel surface plasmon resonance (SPR)-based point of care testing (POCT) approach of microorganism nucleic acids with the guidance of CRISPR enzyme is described, including the application of optical fiber-based detection of trace SARS-CoV2 virus in sewage water on SPR and validation of the plasmonic biosensor for the detection of single-nucleotide mutations in natural water samples.
在新冠疫情后时代，对病原体的长期监测仍然很重要。病原体的快速检测有助于对微生物污染进行准确、便捷的实时监测，并改善疾病管理。在此，描述了一种基于新型表面等离子体共振（SPR）的、在CRISPR酶引导下的微生物核酸即时检测（POCT）方法，包括基于光纤在SPR上检测污水中痕量SARS-CoV2病毒的应用以及对等离激元生物传感器用于检测天然水样中单核苷酸突变的验证。
