National Engineering Research Center of JUNCAO Technology, Fujian, Fuzhou 350002, China; College of Life Science, Fujian Agriculture and Forestry University, Fujian, Fuzhou 350002, China.
Chongqing Key Laboratory for the Development and Utilization of Genuine Medicinal Materials in the Three Gorges Reservoir Area, Chongqing Three Gorge Medical College, Chongqing 404120, China.
Int J Biol Macromol. 2024 Mar;261(Pt 2):129793. doi: 10.1016/j.ijbiomac.2024.129793. Epub 2024 Jan 28.
A water-soluble glycopeptide (named GL-PWQ) with a molecular weight (M) of 2.40 × 10 g/mol was isolated from Ganoderma lucidum fruiting body by hot water extraction, membrane ultrafiltration, and gel column chromatography, which mainly consisted of glucose and galactose. Based on the methylation, FT-IR, 1D, and 2D NMR analysis, the polysaccharide portion of GL-PWQ was identified as a glucogalactan, which was comprised of unsubstituted (1,6-α-Galp, 1,6-β-Glcp, 1,4-β-Glcp) and monosubstituted (1,2,6-α-Galp and 1,3,6-β-Glcp) in the backbone and possible branches that at the O-3 position of 1,3-Glcp and T-Glcp, and the O-2 position of T-Fucp, T-Manp or T-Glcp. The chain conformational study by SEC-MALLS-RI and AFM revealed that GL-PWQ was identified as a highly branched polysaccharide with a polydispersity index of 1.25, and might have compact sphere structures caused by stacked multiple chains. Moreover, the GL-PWQ shows strong anti-oxidative activity in NRK-52E cells. This study provides a theoretical basis for further elucidating the structure-functionality relationships of GL-PWQ and its potential application as a natural antioxidant in pharmacotherapy as well as functional food additives.
一种水溶性糖肽（命名为 GL-PWQ），分子量（M）为 2.40×10g/mol，从灵芝子实体中通过热水提取、膜超滤和凝胶柱层析分离得到，主要由葡萄糖和半乳糖组成。基于甲基化、FT-IR、1D 和 2D NMR 分析，GL-PWQ 的多糖部分被鉴定为葡半乳糖聚糖，由未取代的（1,6-α-Galp、1,6-β-Glcp、1,4-β-Glcp）和单取代的（1,2,6-α-Galp 和 1,3,6-β-Glcp）在主链和可能在 1,3-Glcp 和 T-Glcp 的 O-3 位置以及 T-Fucp、T-Manp 或 T-Glcp 的 O-2 位置的支链组成。通过 SEC-MALLS-RI 和 AFM 进行的链构象研究表明，GL-PWQ 被鉴定为具有高度支化的多糖，其多分散指数为 1.25，可能由于堆叠的多个链而具有紧凑的球体结构。此外，GL-PWQ 在 NRK-52E 细胞中表现出很强的抗氧化活性。这项研究为进一步阐明 GL-PWQ 的结构-功能关系及其作为天然抗氧化剂在药理学治疗以及功能性食品添加剂中的潜在应用提供了理论基础。
