Lin Jer-Young, Liu Yu-Chang, Tseng Yan-Hao, Chan Ming-Tsair, Chang Ching-Chun
Agricultural Biotechnology Research Center, Academia Sinica, Tainan, 71150, Taiwan.
Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, 70101, Taiwan.
Plant Cell Rep. 2024 Feb 10;43(3):61. doi: 10.1007/s00299-024-03150-w.
TALE-based editors provide an alternative way to engineer the organellar genomes in plants. We update and discuss the most recent developments of TALE-based organellar genome editing in plants. Gene editing tools have been widely used to modify the nuclear genomes of plants for various basic research and biotechnological applications. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 editing platform is the most commonly used technique because of its ease of use, fast speed, and low cost; however, it encounters difficulty when being delivered to plant organelles for gene editing. In contrast, protein-based editing technologies, such as transcription activator-like effector (TALE)-based tools, could be easily delivered, expressed, and targeted to organelles in plants via Agrobacteria-mediated nuclear transformation. Therefore, TALE-based editors provide an alternative way to engineer the organellar genomes in plants since the conventional chloroplast transformation method encounters technical challenges and is limited to certain species, and the direct transformation of mitochondria in higher plants is not yet possible. In this review, we update and discuss the most recent developments of TALE-based organellar genome editing in plants.
基于转录激活样效应因子（TALE）的编辑器为改造植物细胞器基因组提供了一种替代方法。我们更新并讨论了基于TALE的植物细胞器基因组编辑的最新进展。基因编辑工具已被广泛用于修改植物的核基因组，以用于各种基础研究和生物技术应用。成簇规律间隔短回文重复序列（CRISPR）/Cas9编辑平台是最常用的技术，因为它使用方便、速度快且成本低；然而，当将其用于植物细胞器的基因编辑时会遇到困难。相比之下，基于蛋白质的编辑技术，如基于转录激活样效应因子（TALE）的工具，可以通过农杆菌介导的核转化轻松递送至植物细胞器、在其中表达并靶向细胞器。因此，基于TALE的编辑器为改造植物细胞器基因组提供了一种替代方法，因为传统的叶绿体转化方法面临技术挑战且仅限于某些物种，而高等植物中线粒体的直接转化尚不可能。在本综述中，我们更新并讨论了基于TALE的植物细胞器基因组编辑的最新进展。
