Canadian Research Institute for Food Safety (CRIFS), University of Guelph, Guelph, ON, N1G 2W1, Canada.
Bayview Secondary School, 10077 Bayview Ave, Richmond Hill, ON, L4C 2L4, Canada.
BMC Genom Data. 2024 Feb 15;25(1):18. doi: 10.1186/s12863-024-01204-2.
Vibrio cholerae is an enteric pathogen that poses a significant threat to global health. It causes severe dehydrating diarrheal disease cholera in humans. V. cholerae could be acquired either from consuming contaminated seafood or direct contact with polluted waters. As part of a larger program that assesses the microbial community profile in aquatic systems, V. cholerae strain NB-183 was isolated and characterized using a combination of culture- and whole-genome sequencing-based approaches.
Here we report the assembled and annotated whole-genome sequence of a V. cholerae strain NB-183 isolated from a recreational freshwater lake in Ontario, Canada. The genome was sequenced using short-read Illumina systems. The whole-genome sequencing yielded 4,112,549 bp genome size with 99 contigs with an average genome coverage of 96× and 47.42% G + C content. The whole genome-based comparison, phylogenomic and gene repertoire indicates that this strain harbors multiple virulence genes and biosynthetic gene clusters. This genome sequence and its associated datasets provided in this study will be an indispensable resource to enhance the understanding of the functional, ecological, and evolutionary dynamics of V. cholerae.
霍乱弧菌是一种肠道病原体，对全球健康构成重大威胁。它会导致人类发生严重的脱水性腹泻病霍乱。人类可以通过食用受污染的海鲜或直接接触受污染的水来感染霍乱弧菌。作为评估水生系统微生物群落特征的更大计划的一部分，使用基于培养和全基因组测序的组合方法分离和表征了霍乱弧菌 NB-183 菌株。
在这里，我们报告了从加拿大安大略省一个休闲淡水湖中分离出的霍乱弧菌 NB-183 菌株的组装和注释的全基因组序列。该基因组是使用短读长 Illumina 系统测序的。全基因组测序得到了 4112549 bp 的基因组大小，有 99 个基因组，平均基因组覆盖率为 96×，G+C 含量为 47.42%。基于全基因组的比较、系统发育和基因库表明，该菌株含有多个毒力基因和生物合成基因簇。本研究提供的这个基因组序列及其相关数据集将成为增强对霍乱弧菌功能、生态和进化动态理解的不可或缺的资源。
