Youté O'Neal Dorsel, Domngang Noche Christelle, Tamatcho Kweyang Blandine Pulcherie, Kougang Esther Guladys, Fotsing Kwetche Pierre René
Laboratory of Microbiology, Université des Montagnes Teaching Hospital, Bangangté-Cameroon.
School of Biomedical Sciences, Higher Institute of Health Sciences, Université des Montagnes, Bangangté-Cameroon.
Heliyon. 2024 Feb 6;10(4):e25647. doi: 10.1016/j.heliyon.2024.e25647. eCollection 2024 Feb 29.
Many infections in healthcare facilities are associated with the microbiological quality of the work environment, generally due to poor sanitation.
In order to evaluate the effectiveness of a decontamination protocol (cleaning + disinfection) applied at the "Université des Montagnes" Teaching Hospital, the present study assessed the variation of bacterial loads on surfaces subsequent to decontamination. Susceptibility of bacteria to disinfectants was also evaluated in the same frame.
This work was conducted with an adjusted bacterial detection/enumeration and susceptibility test protocols and standard bacterial identification protocols. Sampling on surfaces was performed by wet swabbing before cleaning, between cleaning and disinfection and after disinfection.
Major findings revealed the predominance of (75.5%) on target surfaces. High bacterial loads recorded on these surfaces before decontamination became undetectable after cleaning with the detergent "Pax lemon". The majority of isolates (98%) were susceptible to the disinfectants tested, (Surfanios® 0.25% and sodium hypochlorite 0.12%).
Overall, these findings indicated process effectiveness on the subjected bacterial populations and suggest the use of either Surfanios® (0.25%) or sodium hypochlorite (0.12%) for work surfaces hygiene, justifying the use of these products in this department for surface decontamination. Also, cleaning with the detergent "Pax lemon" and disinfection with sodium hypochlorite may be sufficient for the types of surfaces subjected in the present research.
医疗机构中的许多感染都与工作环境的微生物质量有关，这通常是由于卫生条件差所致。
为了评估在“Université des Montagnes”教学医院应用的去污方案（清洁 + 消毒）的有效性，本研究评估了去污后表面细菌载量的变化。同时还在相同背景下评估了细菌对消毒剂的敏感性。
本研究采用了经过调整的细菌检测/计数和药敏试验方案以及标准细菌鉴定方案。在清洁前、清洁与消毒之间以及消毒后，通过湿拭法对表面进行采样。
主要研究结果显示目标表面上（75.5%）占主导地位。在用“Pax lemon”洗涤剂清洁之前，这些表面上记录的高细菌载量在清洁后变得无法检测到。大多数分离株（98%）对所测试的消毒剂（0.25%的Surfanios®和0.12%的次氯酸钠）敏感。
总体而言，这些结果表明该过程对受试细菌群体有效，并建议使用0.25%的Surfanios®或0.12%的次氯酸钠进行工作表面卫生处理，证明在该科室使用这些产品进行表面去污是合理的。此外，用“Pax lemon”洗涤剂清洁并用次氯酸钠消毒可能足以应对本研究中所涉及的表面类型。
