Department of Pharmaceutical Botany and Pharmacognosy, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Marie Curie-Skłodowska 9, 85-094 Bydgoszcz, Poland.
Department of Pathophysiology, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Marie Curie-Skłodowska 9, 85-094 Bydgoszcz, Poland.
Int J Mol Sci. 2024 Feb 7;25(4):2033. doi: 10.3390/ijms25042033.
Fruits are very important dietary components and a source of biologically active compounds used in nutritional pharmacology. Particularly due to the presence of polyphenolic compounds, fruits play an important role in the prevention of diseases of civilization. Therefore, it is important to study the phytochemicals and biological activity of fruits, especially those with a long-standing use in ethnomedicine. In this study, we determined the chemical profile and biological activity of a methanolic extract of the fruits. Amongst nine polyphenols studied, only chlorogenic acid, protocatechuic acid, and eleutheroside E have been detected. The extract showed a weak anti-hyaluronidase activity from bovine testicular in a range of 9.06-37.70% and quite high for human serum hyaluronidase from children diagnosed with acute leukemia in a range of 76-86%. A weak anti-tyrosinase activity was obtained in a range of 2.94-12.46%. Moreover, the extract showed antioxidant properties against DPPH radical, ABTS radical, and O. In addition, the antioxidant activity of the extract was evaluated by FRAP assay and Fe ion chelation assay. These preliminary studies partially justify the traditional use of the plant in inflammatory- and immune-related diseases, in which hyaluronidase and free radicals can participate. A difference in human serum hyaluronidase inhibition may result from the inter-patient variability. Regardless of that, the results mean that polyphenolic compounds may stimulate activity of hyaluronidase, as well as to protect cells from the oxidative damages. However, further studies in ex vivo and in vivo models are needed, including blood isolated from a larger number of patients.
水果是非常重要的膳食成分，也是营养药理学中生物活性化合物的来源。特别是由于多酚类化合物的存在，水果在预防文明病方面发挥着重要作用。因此，研究水果的植物化学物质和生物活性非常重要，尤其是那些在民族医学中使用历史悠久的水果。在这项研究中，我们确定了水果甲醇提取物的化学特征和生物活性。在所研究的九种多酚中，仅检测到绿原酸、原儿茶酸和刺五加苷 E。该提取物在牛睾丸透明质酸酶中显示出较弱的抑制活性，范围为 9.06-37.70%，在儿童急性白血病来源的人血清透明质酸酶中显示出相当高的活性，范围为 76-86%。在酪氨酸酶中获得了较弱的抑制活性，范围为 2.94-12.46%。此外，该提取物还显示出对 DPPH 自由基、ABTS 自由基和 O 的抗氧化特性。此外，还通过 FRAP 测定法和 Fe 离子螯合测定法评估了提取物的抗氧化活性。这些初步研究部分证实了植物在炎症和免疫相关疾病中的传统用途，其中透明质酸酶和自由基可以参与其中。人血清透明质酸酶抑制的差异可能是由于患者间的个体差异所致。尽管如此，这些结果意味着多酚类化合物可能刺激透明质酸酶的活性，以及保护细胞免受氧化损伤。然而，仍需要进行离体和在体模型的进一步研究，包括从更多患者中分离的血液。
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