Kitahara M, Ishii K, Kumada Y, Shiraishi T, Furuta T, Miwa T, Kawaharada H, Watanabe K
J Antibiot (Tokyo). 1985 Aug;38(8):972-6. doi: 10.7164/antibiotics.38.972.
Strain A-347, an actinomycete isolated from a soil sample, was found to produce a new antimetabolite, 7-hydroxyguanine. The aerial mycelium formed spiral spore chains with spiny spore surface. The chemical composition of strain A-347 indicated that it was an actinomycete of cell wall Type I. From its morphological, cultural, physiological characteristics and direct comparison with the type culture, this strain was classified as Streptomyces purpurascens. 7-Hydroxyguanine was purified from the broth filtrate by ion exchange chromatography and crystallized from hot 2 M NH4OH. 7-Hydroxyguanine inhibited the growth of experimental tumors such as L1210 leukemia.
从一份土壤样本中分离出的放线菌菌株A-347被发现能产生一种新的抗代谢物，即7-羟基鸟嘌呤。气生菌丝形成螺旋状孢子链，孢子表面有刺。菌株A-347的化学成分表明它是细胞壁I型的放线菌。根据其形态、培养、生理特征以及与模式菌株的直接比较，该菌株被归类为紫色链霉菌。通过离子交换色谱法从发酵液滤液中纯化出7-羟基鸟嘌呤，并从热的2M氢氧化铵中结晶出来。7-羟基鸟嘌呤能抑制诸如L1210白血病等实验性肿瘤的生长。
