Ikenami M, Yamazaki M
Br J Cancer. 1985 Oct;52(4):575-81. doi: 10.1038/bjc.1985.229.
Previous studies showed that murine polymorphonuclear leukocytes (PMNs) lyze tumour cells in the presence of wheat germ agglutinin or actinomycin D. This paper reports studies on whether a soluble factor participates in PMN-mediated cytolysis dependent on lectin or a chemotherapeutic drug. Tumour lysis was observed with supernatants from PMN cocultured with wheat germ agglutinin or actinomycin D. The supernatant from cultures of PMNs alone was not cytotoxic, but addition of these agents to the supernatant induced tumour lysis. PMNs released a soluble factor spontaneously into the medium and cytolysis was induced by a combination of this factor and wheat germ agglutinin or actinomycin D. This factor was not an oxygen metabolite, but a protein with a molecular weight of approximately 100 K daltons. These results suggest that a soluble factor(s) from PMNs participates in tumour killing in cooperation with appropriate reagents.
先前的研究表明，小鼠多形核白细胞（PMN）在存在麦胚凝集素或放线菌素D的情况下可裂解肿瘤细胞。本文报道了关于一种可溶性因子是否参与依赖凝集素或化疗药物的PMN介导的细胞溶解的研究。在用麦胚凝集素或放线菌素D共培养的PMN的上清液中观察到肿瘤溶解。单独培养PMN的上清液没有细胞毒性，但向上清液中添加这些试剂可诱导肿瘤溶解。PMN自发地向培养基中释放一种可溶性因子，这种因子与麦胚凝集素或放线菌素D共同作用可诱导细胞溶解。该因子不是氧代谢产物，而是一种分子量约为100千道尔顿的蛋白质。这些结果表明，来自PMN的一种或多种可溶性因子与适当的试剂协同参与肿瘤杀伤。
