Yamazaki M, Ikenami M, Sugiyama T
Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa.
Jpn J Cancer Res. 1988 Nov;79(11):1217-23. doi: 10.1111/j.1349-7006.1988.tb01547.x.
Murine inflammatory polymorphonuclear leukocytes (PMN) and inflammatory ascitic fluid were found to contain cytotoxin. This cytotoxin was not oxygen metabolites, but a protein with a molecular weight of 70 kilodaltons as determined by high-performance liquid chromatography. However, the cytolytic activity was completely neutralized by anti-murine tumor necrosis factor (TNF) antibody, suggesting that PMN cytotoxin is a TNF-like molecule or TNF itself. Cytolytic heterogeneity of PMN was also demonstrated: immature PMN obtained from ascitic fluid 6 hr after caseinate injection showed stronger cytotoxicity than mature PMN obtained 3 hr after the injection. However, mature PMN (3 hr) contained more cytotoxin than immature PMN (6 hr). This discrepancy may be due to differences in the abilities of these PMN to synthesize and release cytotoxin. These results indicate that not only macrophages, but also inflammatory PMN can produce a TNF-like cytotoxin and that cytolytic heterogeneity of PMN exists in tumor killing.
研究发现，小鼠炎性多形核白细胞（PMN）和炎性腹水含有细胞毒素。这种细胞毒素并非氧代谢产物，而是一种经高效液相色谱测定分子量为70千道尔顿的蛋白质。然而，其细胞溶解活性被抗小鼠肿瘤坏死因子（TNF）抗体完全中和，这表明PMN细胞毒素是一种TNF样分子或TNF本身。还证实了PMN的细胞溶解异质性：在酪蛋白注射后6小时从腹水中获得的未成熟PMN显示出比注射后3小时获得的成熟PMN更强的细胞毒性。然而，成熟PMN（3小时）所含细胞毒素比未成熟PMN（6小时）更多。这种差异可能是由于这些PMN合成和释放细胞毒素的能力不同所致。这些结果表明，不仅巨噬细胞，而且炎性PMN也能产生TNF样细胞毒素，并且PMN的细胞溶解异质性存在于肿瘤杀伤过程中。
