含有来自大肠杆菌rrn B顺反子核糖体DNA序列的质粒的分离与限制酶切图谱分析。
Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli.
作者信息
Palmer M L, Raker M A, Kennedy P J, Young J W, Barnes W M, Rodriguez R L, Noller H F
出版信息
Mol Gen Genet. 1979 May 4;172(2):171-8. doi: 10.1007/BF00268279.
PMID:384145
Abstract
Recombinant plasmids containing the entire 16S RNA gene from the rrn B cistron of E. coli inserted in Col E1 and pBR322 plasmid vectors have been constructed. These plasmids have been mapped using several restriction endonucleases as well as by DNA-RNA hybridization. These maps reveal previously undetected restriction sites in the rrn B cistron and in Col E1 plasmid DNA.
摘要
已构建出含有来自大肠杆菌rrnB顺反子的完整16S RNA基因并插入到Col E1和pBR322质粒载体中的重组质粒。这些质粒已通过几种限制性内切核酸酶以及DNA - RNA杂交进行了图谱绘制。这些图谱揭示了rrnB顺反子和Col E1质粒DNA中以前未检测到的限制性位点。
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