Han Qibin, Bai Lang, Qian Yinhua, Zhang Xiaoyu, Wang Juan, Zhou Jing, Cui Wenguo, Hao Yuefeng, Yang Xing
Department of Orthopedics, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University, Suzhou 215008, P.R. China.
Department of Orthopedics, Kunshan Hospital of Traditional Chinese Medicine, Suzhou 215300, P.R. China.
Regen Biomater. 2024 Jan 30;11:rbae007. doi: 10.1093/rb/rbae007. eCollection 2024.
Tendinopathy is a common disorder that causes local dysfunction and reduces quality of life. Recent research has indicated that alterations in the inflammatory microenvironment play a vital role in the pathogenesis of tendinopathy. Herein, injectable methacrylate gelatin (GelMA) microspheres (GM) were fabricated and loaded with heparin-dopamine conjugate (HDC) and hepatocyte growth factor (HGF). GM@HDC@HGF were designed to balance the inflammatory microenvironment by inhibiting oxidative stress and inflammation, thereby regulating extracellular matrix (ECM) metabolism and halting tendon degeneration. Combining growth factors with heparin was expected to improve the encapsulation rate and maintain the long-term efficacy of HGF. In addition, the catechol groups on dopamine have adhesion and antioxidant properties, allowing potential attachment at the injured site, and better function synergized with HGF. GM@HDC@HGF injected in rat Achilles tendinopathy (AT) models significantly down-regulated oxidative stress and inflammation, and ameliorated ECM degradation. In conclusion, the multifunctional platform developed presents a promising alternative for the treatment of tendinopathy.
肌腱病是一种常见的疾病，会导致局部功能障碍并降低生活质量。最近的研究表明，炎症微环境的改变在肌腱病的发病机制中起着至关重要的作用。在此，制备了可注射的甲基丙烯酸明胶（GelMA）微球（GM），并负载了肝素-多巴胺共轭物（HDC）和肝细胞生长因子（HGF）。GM@HDC@HGF旨在通过抑制氧化应激和炎症来平衡炎症微环境，从而调节细胞外基质（ECM）代谢并阻止肌腱退变。将生长因子与肝素结合有望提高包封率并维持HGF的长期疗效。此外，多巴胺上的儿茶酚基团具有粘附和抗氧化特性，能够在损伤部位实现潜在附着，并与HGF更好地发挥协同作用。在大鼠跟腱病（AT）模型中注射GM@HDC@HGF可显著下调氧化应激和炎症，并改善ECM降解。总之，所开发的多功能平台为肌腱病的治疗提供了一种有前景的替代方案。
