新型抗肿瘤抗生素terpentecin的分离与鉴定

Isolation and characterization of terpentecin, a new antitumor antibiotic.

作者信息

Tamamura T, Sawa T, Isshiki K, Masuda T, Homma Y, Inuma H, Naganawa H, Hamada M, Takeuchi T, Umezawa H

出版信息

J Antibiot (Tokyo). 1985 Dec;38(12):1664-9. doi: 10.7164/antibiotics.38.1664.

DOI:10.7164/antibiotics.38.1664

Abstract

A new antitumor antibiotic, terpentecin was isolated from the culture broth of strain MF730-N6. Strain MF730-N6, isolated from soil, was found to belong to the genus Kitasatosporia. The antibiotic was extracted with chloroform, purified by column chromatography using silica gel and Diaion HP-20 successively, and finally purified by high performance reverse-phase thin layer chromatography. The molecular formula of terpentecin was determined to be C20H28O6 (molecular weight, 364). The antibiotic inhibited the growth of Gram-positive and Gram-negative bacteria, and prolonged the survival period of mice bearing leukemia L-1210, P388 and Ehrlich ascites carcinoma.

摘要

一种新的抗肿瘤抗生素——特戊菌素，是从菌株MF730-N6的培养液中分离得到的。菌株MF730-N6从土壤中分离得到，被发现属于北里孢菌属。该抗生素用氯仿萃取，先后通过硅胶柱色谱和Diaion HP-20进行纯化，最后通过高效反相薄层色谱进行纯化。特戊菌素的分子式确定为C20H28O6（分子量为364）。该抗生素能抑制革兰氏阳性菌和革兰氏阴性菌的生长，并延长携带白血病L-1210、P388和艾氏腹水癌的小鼠的生存期。