大肠杆菌K12中malB区域的结构。II. malE、F、G操纵子的遗传图谱。
Structure of the malB region in Escherichia coli K12. II. Genetic map of the malE,F,G operon.
作者信息
Silhavy T J, Brickman E, Bassford P J, Casadaban M J, Shuman H A, Schwartz V, Guarente L, Schwartz M, Beckwith J R
出版信息
Mol Gen Genet. 1979 Jul 24;174(3):249-59. doi: 10.1007/BF00267797.
PMID:384167
Abstract
Starting with a strain containing a malK-lacZ fusion, a series of lambda plaque-forming phages which carry varying amounts of the malE,F operon have been isolated. We have used these phages to construct a deletion map of the malE,F operon. The construction of this deletion map has led to the identification of a new gene, malG. The malG gene is located distal to malF. The malG gene product is a protein required for the active transport of maltose and maltodextrins.
摘要
从含有malK-lacZ融合基因的菌株开始，已分离出一系列携带不同量malE,F操纵子的λ噬菌斑形成噬菌体。我们利用这些噬菌体构建了malE,F操纵子的缺失图谱。该缺失图谱的构建导致鉴定出一个新基因malG。malG基因位于malF的远端。malG基因产物是麦芽糖和麦芽糊精主动运输所需的一种蛋白质。
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