Asagoshi Yuna, Hitomi Eri, Nakamura Noriko, Takeda Seiji
Department of Agricultural and Life Science, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Shimogamo Hangi-cho, Sakyo-ku, Kyoto 606-8522, Japan.
Research Institute, Suntory Global Innovation Center Ltd., Seikadai 8-1-1, Seika-cho, Kyoto 619-0284, Japan.
Plant Biotechnol (Tokyo). 2023 Dec 25;40(4):283-288. doi: 10.5511/plantbiotechnology.23.0708a.
Rose is a major ornamental plant, and a lot of cultivars with attractive morphology, color and scent have been generated by classical breeding. Recent progress of genetic modification produces a novel cultivar with attractive features. In both cases, a major problem is the gene-flow from cultivated or genetically modified (GM) plants to wild species, causing reduction of natural population. To investigate whether gene-flow occurs in wild species, molecular analysis with DNA markers with higher efficient technique is useful. Here we investigated the gene-flow from cultivated roses (×) to wild rose species planted in close distance in the field. The overlapping flowering periods and visiting insects suggest that pollens were transported by insects between wild and cultivated roses. We examined the germination ratio of seeds from wild species, and extracted DNA and checked with and () DNA markers to detect transposon insertions. Using two markers, we successfully detected the outcross between wild and cultivated roses. For higher efficiency, we established a bulking method, where DNA, leaves or embryos were pooled, enabling us to that check the outcross of many plants. Our results suggest that wild species and garden cultivars can cross in close distance, so that they should be planted in distance, and checked the outcross with multiple DNA markers.
玫瑰是一种主要的观赏植物，通过传统育种已培育出许多形态、颜色和香味诱人的品种。基因改造的最新进展培育出了具有诱人特征的新品种。在这两种情况下，一个主要问题是栽培植物或转基因植物的基因流向野生物种，导致自然种群数量减少。为了研究野生物种中是否发生基因流动，采用高效技术的DNA标记进行分子分析是有用的。在这里，我们研究了栽培玫瑰（×）向田间近距离种植的野生玫瑰物种的基因流动。重叠的花期和访花昆虫表明花粉是通过昆虫在野生玫瑰和栽培玫瑰之间传播的。我们检测了野生玫瑰种子的萌发率，提取了DNA，并使用[具体标记名称1]和[具体标记名称2]（[标记类型]）DNA标记检查转座子插入情况。使用这两种标记，我们成功检测到了野生玫瑰和栽培玫瑰之间的杂交。为了提高效率，我们建立了一种混合法，即将DNA、叶片或胚汇集在一起，使我们能够检查许多植株的杂交情况。我们的结果表明，野生玫瑰物种和园艺品种在近距离内可以杂交，因此它们应该间隔种植，并用多种DNA标记检查杂交情况。
