Dong Kairu, Liu Wen-Che, Su Yuyan, Lyu Yidan, Huang Hao, Zheng Nenggan, Rogers John A, Nan Kewang
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
National Key Laboratory of Advanced Drug Delivery and Release Systems, Zhejiang University, Hangzhou 310058, China.
BME Front. 2023 Dec 7;4:0034. doi: 10.34133/bmef.0034. eCollection 2023.
Millimeter-scale animals such as , larvae, zebrafish, and bees serve as powerful model organisms in the fields of neurobiology and neuroethology. Various methods exist for recording large-scale electrophysiological signals from these animals. Existing approaches often lack, however, real-time, uninterrupted investigations due to their rigid constructs, geometric constraints, and mechanical mismatch in integration with soft organisms. The recent research establishes the foundations for 3-dimensional flexible bioelectronic interfaces that incorporate microfabricated components and nanoelectronic function with adjustable mechanical properties and multidimensional variability, offering unique capabilities for chronic, stable interrogation and stimulation of millimeter-scale animals and miniature tissue constructs. This review summarizes the most advanced technologies for electrophysiological studies, based on methods of 3-dimensional flexible bioelectronics. A concluding section addresses the challenges of these devices in achieving freestanding, robust, and multifunctional biointerfaces.
毫米级动物，如 幼虫、斑马鱼和蜜蜂，是神经生物学和神经行为学领域强大的模式生物。存在多种用于记录来自这些动物的大规模电生理信号的方法。然而，由于其刚性结构、几何约束以及与软体生物集成时的机械不匹配，现有方法往往缺乏实时、不间断的研究。最近的研究为三维柔性生物电子接口奠定了基础，该接口将微制造组件和纳米电子功能与可调节的机械性能和多维变异性相结合，为毫米级动物和微型组织构建体的长期、稳定的询问和刺激提供了独特的能力。本综述基于三维柔性生物电子学方法总结了电生理研究的最先进技术。结论部分讨论了这些设备在实现独立、坚固和多功能生物接口方面所面临的挑战。
