Lingamgunta Swetha, Xiao Yao, Choi Heungjae, Christie Graham, Fruk Ljiljana
Department of Chemical Engineering and Biotechnology, University of Cambridge Cambridge UK
School of Engineering, Cardiff University Wales UK.
RSC Adv. 2024 Mar 11;14(12):8331-8340. doi: 10.1039/d3ra07543e. eCollection 2024 Mar 6.
The ever-increasing risks posed by antibiotic-resistant bacteria have stimulated considerable interest in the development of novel antimicrobial strategies, including the use of nanomaterials that can be activated on demand and result in irreversible damage to pathogens. Microwave electric field-assisted bactericidal effects on representative Gram-negative and Gram-positive bacterial strains were achieved in the presence of hybrid polydopamine-silver nanoparticles (PDA-Ag NPs) under low-power microwave irradiation using a resonant cavity (1.3 W, 2.45 GHz). A 3-log reduction in the viability of bacterial populations was observed within 30 minutes which was attributed to the attachment of PDA-Ag NPs and associated membrane disruption in conjunction with the production of intra-bacterial reactive oxygen species (ROS). A synergistic effect between PDA and Ag has been demonstrated whereby PDA acts both as an Ag NP carrier and a microwave enhancer. These properties together with the remarkable adhesivity of PDA are opening a route to design of antibacterial adhesives and surface coatings for prevention of biofilm formation.
抗生素耐药细菌带来的风险不断增加，激发了人们对开发新型抗菌策略的浓厚兴趣，包括使用可按需激活并对病原体造成不可逆损害的纳米材料。在使用谐振腔（1.3 W，2.45 GHz）进行低功率微波辐照的情况下，在混合聚多巴胺 - 银纳米颗粒（PDA - Ag NPs）存在下，实现了对代表性革兰氏阴性和革兰氏阳性细菌菌株的微波电场辅助杀菌效果。在30分钟内观察到细菌群体活力降低了3个对数，这归因于PDA - Ag NPs的附着以及相关的膜破坏，同时还产生了细菌内活性氧（ROS）。已证明PDA和Ag之间存在协同效应，其中PDA既作为Ag NP载体又作为微波增强剂。这些特性与PDA显著的粘附性一起，为设计用于预防生物膜形成的抗菌粘合剂和表面涂层开辟了一条途径。
