Department of Medicine, Division of Rheumatology, Lowance Center for Human Immunology, Emory University, Atlanta, GA, USA; Emory Autoimmunity Center of Excellence, Emory University, Atlanta, GA, USA.
Department of Medicine, Division of Rheumatology, Lowance Center for Human Immunology, Emory University, Atlanta, GA, USA; Emory Autoimmunity Center of Excellence, Emory University, Atlanta, GA, USA.
Semin Immunol. 2024 Mar;72:101875. doi: 10.1016/j.smim.2024.101875. Epub 2024 Mar 14.
The integration of multi-'omic datasets into complex systems-wide assessments has become a mainstay in immunologic investigation. This focus on high-dimensional data collection and analysis was on full display in the investigation of COVID-19, the respiratory illness resulting from infection by the novel coronavirus SARS-CoV-2. Particularly in the area of B cell biology, tremendous efforts in both cellular and serologic investigation have resulted in an increasingly detailed mapping of the coordinated effector, memory, and antibody secreting cell responses that underpin the development of humoral immunity in response to primary viral infection. Further, the rapid development and deployment of effective vaccines has allowed for the assessment of developing memory responses across a wide variety of immune contexts, including in patients with compromised immune function. The result has been a period of rapid gains in the understanding of B cell biology unrestricted to the study of COVID-19. Here, we outline the systems-level technologies that have been routinely implemented in these investigations throughout the pandemic, and discuss how their use has led to clear and applicable gains in pursuance of the amelioration of human infectious disease and beyond.
多组学数据集的整合已成为复杂系统全面评估中的主要手段，这在免疫研究中得到了充分体现。对 COVID-19 的研究就是一个很好的例子，COVID-19 是由新型冠状病毒 SARS-CoV-2 感染引起的呼吸道疾病。特别是在 B 细胞生物学领域，细胞和血清学研究的巨大努力已经对协调的效应器、记忆和抗体分泌细胞反应进行了越来越详细的描绘，这些反应是针对原发性病毒感染产生体液免疫的基础。此外，快速开发和部署有效的疫苗，使得人们能够在广泛的免疫背景下评估记忆反应的发展，包括在免疫功能受损的患者中。其结果是，人们对 B 细胞生物学的理解在 COVID-19 研究之外也取得了快速进展。在这里，我们概述了在整个大流行期间这些研究中常规实施的系统级技术，并讨论了它们的使用如何在改善人类传染病等方面取得了明确和适用的进展。
