Wang Lijing, Park Lauren, Wu Weisheng, King Dana, Vega-Medina Alexis, Raven Frank, Martinez Jessy, Ensing Amy, McDonald Katherine, Yang Zhongying, Jiang Sha, Aton Sara J
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Bioinformatics Core, Biomedical Research Core Facilities, University of Michigan, Ann Arbor, MI 48109, USA.
iScience. 2024 Mar 4;27(4):109408. doi: 10.1016/j.isci.2024.109408. eCollection 2024 Apr 19.
Post-learning sleep is essential for hippocampal memory processing, including contextual fear memory consolidation. We labeled context-encoding engram neurons in the hippocampal dentate gyrus (DG) and assessed reactivation of these neurons after fear learning. Post-learning sleep deprivation (SD) selectively disrupted reactivation of inferior blade DG engram neurons, linked to SD-induced suppression of neuronal activity in the inferior, but not superior DG blade. Subregion-specific spatial profiling of transcripts revealed that transcriptomic responses to SD differed greatly between hippocampal CA1, CA3, and DG inferior blade, superior blade, and hilus. Activity-driven transcripts, and those associated with cytoskeletal remodeling, were selectively suppressed in the inferior blade. Critically, learning-driven transcriptomic changes differed dramatically between the DG blades and were absent from all other regions. Together, these data suggest that the DG is critical for sleep-dependent memory consolidation, and that the effects of sleep loss on the hippocampus are highly subregion-specific.
学习后的睡眠对于海马体记忆处理至关重要，包括情境恐惧记忆巩固。我们标记了海马齿状回（DG）中编码情境的记忆痕迹神经元，并评估了恐惧学习后这些神经元的重新激活情况。学习后睡眠剥夺（SD）选择性地破坏了齿状回下叶片记忆痕迹神经元的重新激活，这与SD诱导的下叶片而非上叶片DG神经元活动抑制有关。转录本的亚区域特异性空间分析显示，海马CA1、CA3以及DG下叶片、上叶片和海马体 hilus 对SD的转录组反应差异很大。活动驱动的转录本以及与细胞骨架重塑相关的转录本在下叶片中被选择性抑制。至关重要的是，学习驱动的转录组变化在DG叶片之间差异巨大，而在所有其他区域均不存在。总之，这些数据表明DG对于依赖睡眠的记忆巩固至关重要，并且睡眠剥夺对海马体的影响具有高度的亚区域特异性。
