Flower Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China.
Genes (Basel). 2024 Feb 23;15(3):277. doi: 10.3390/genes15030277.
The flower's color is regarded as one of the most outstanding features of the rose. Byhouwer, an endemic and critically endangered decaploid wild rose species, is abundant in phenotypic diversity, especially in flower color variation, from white to different degrees of pink. The mechanism underlying this variation, e.g., the level of petal-color-related genes, is worth probing. Seven candidate reference genes for qRT-PCR analysis, including tubulin α chain (), glyceraldehyde-3-phosphate dehydrogenase (), histone H2B (), eukaryotic translation elongation factor 1-α (), 60S ribosomal protein (), eukaryotic translation initiation factor 1-α (), and aquaporins (), were detected from the transcriptome datasets of full blooming flowers of white-petaled and pink-petaled individuals, and their expression stabilities were evaluated through qRT-PCR analysis. According to stability rankings analysis, showed the highest stability and could be chosen as the most suitable reference gene. Moreover, the reliability of was demonstrated via qRT-PCR analysis of six petal-color-related target genes, the expression patterns of which, through normalization, were found to be consistent with the findings of transcriptome analysis. The result provides an optimal reference gene for exploring the expression level of petal-color-related genes in , which will accelerate the dissection of petal-color-variation mechanisms in .
花色被认为是玫瑰最重要的特征之一。原生濒危的 10 倍体野生玫瑰(Byhouwer)品种丰富，表型多样，花色尤其多变，从白色到不同程度的粉色。这种变异的机制，如花色相关基因的水平，值得探究。本研究从白色花瓣和粉色花瓣个体的完全盛开花朵的转录组数据集中检测到了用于 qRT-PCR 分析的 7 个候选参考基因，包括微管蛋白α链()、甘油醛-3-磷酸脱氢酶()、组蛋白 H2B()、真核翻译延伸因子 1-α()、60S 核糖体蛋白()、真核翻译起始因子 1-α()和水通道蛋白()，并通过 qRT-PCR 分析评估了它们的表达稳定性。根据稳定性排名分析，()表现出最高的稳定性，可作为最适合的参考基因。此外，通过对 6 个花色相关靶基因的 qRT-PCR 分析验证了的可靠性，通过归一化后发现其表达模式与转录组分析结果一致。该结果为研究花色相关基因在 Byhouwer 中的表达水平提供了一个最优的参考基因，将加速解析花色变异机制。
