Buratta Sandra, Latella Raffaella, Chiaradia Elisabetta, Salzano Anna Maria, Tancini Brunella, Pellegrino Roberto Maria, Urbanelli Lorena, Cerrotti Giada, Calzoni Eleonora, Alabed Husam B R, De Pascale Sabrina, Lugini Luana, Federici Cristina, Scaloni Andrea, Emiliani Carla
Department of Chemistry, Biology and Biotechnology, University of Perugia, 06100 Perugia, Italy.
Department of Veterinary Medicine, University of Perugia, 06100 Perugia, Italy.
Foods. 2024 Mar 8;13(6):835. doi: 10.3390/foods13060835.
Edible plant and fruit-derived nanovesicles (NVs) are membrane-enclosed particles with round-shape morphology and signaling functions, which resemble mammalian cell-derived extracellular vesicles. These NVs can transmit cross-kingdom signals as they contain bioactive molecules and exert biological effects on mammalian cells. Their properties and stability in the gastrointestinal tract suggest NVs as a promising nutraceutical tool. In this study, we have demonstrated for the first time the presence of NVs in olive vegetation water (OVW), a waste by-product generated during olive oil production. Biophysical characterization by scanning electron microscopy, cryo-transmission electron microscopy, and nanoparticle tracking analysis revealed the presence in OVW of NVs having size and morphology similar to that of vesicles isolated from edible plants. Integrated lipidomic, metabolomic, and proteomic analyses showed that OVW-NVs carry a set of lipids, metabolites and proteins which have recognized antioxidant and anti-inflammatory activities. The nature of biomolecules identified in OVW-NVs suggests that these vesicles could exert beneficial effects on mammalian cells and could be used in the nutraceutical and food industries. The successful isolation of OVW-NVs and the characterization of their features strengthen the idea that agricultural waste might represent a source of NVs having features similar to NVs isolated from edible plants/fruits.
可食用植物和水果衍生的纳米囊泡（NVs）是具有圆形形态和信号功能的膜包裹颗粒，类似于哺乳动物细胞衍生的细胞外囊泡。这些NVs可以传递跨界信号，因为它们含有生物活性分子并对哺乳动物细胞产生生物学效应。它们在胃肠道中的性质和稳定性表明NVs是一种有前途的营养保健工具。在本研究中，我们首次证明了橄榄油生产过程中产生的废弃副产品橄榄植被水（OVW）中存在NVs。通过扫描电子显微镜、冷冻透射电子显微镜和纳米颗粒跟踪分析进行的生物物理表征揭示了OVW中存在大小和形态与从可食用植物中分离的囊泡相似的NVs。综合脂质组学、代谢组学和蛋白质组学分析表明，OVW-NVs携带一组具有公认抗氧化和抗炎活性的脂质、代谢物和蛋白质。在OVW-NVs中鉴定出的生物分子的性质表明，这些囊泡可能对哺乳动物细胞产生有益影响，并可用于营养保健和食品工业。成功分离OVW-NVs并对其特征进行表征强化了这样一种观点，即农业废弃物可能是具有与从可食用植物/水果中分离的NVs相似特征的NVs的来源。
