Bandzerewicz Aleksandra, Howis Joanna, Wierzchowski Kamil, Slouf Miroslav, Hodan Jiri, Denis Piotr, Gołofit Tomasz, Pilarek Maciej, Gadomska-Gajadhur Agnieszka
Faculty of Chemistry, Warsaw University of Technology, Warsaw, Poland.
Faculty of Chemical and Process Engineering, Warsaw University of Technology, Warsaw, Poland.
Front Bioeng Biotechnol. 2024 Mar 15;12:1332290. doi: 10.3389/fbioe.2024.1332290. eCollection 2024.
Biomaterials containing citric acid as a building unit show potential for use as blood vessel and skin tissue substitutes. The success in commercializing implants containing a polymer matrix of poly(1,8-octanediol citrate) provides a rationale for exploring polycitrates based on other diols. Changing the aliphatic chain length of the diol allows functional design strategies to control the implant's mechanical properties, degradation profile and surface energy. In the present work, poly(1,2-ethanediol citrate) was synthesized and used as an additive to polylactide in the electrospinning process. It was established that the content of polycitrate greatly influences the nonwovens' properties: an equal mass ratio of polymers resulted in the best morphology. The obtained nonwovens were characterized by surface hydrophilicity, tensile strength, and thermal properties. L929 cell cultures were carried out on their surface. The materials were found to be non-cytotoxic and the degree of porosity was suitable for cell colonization. On the basis of the most important parameters for assessing the condition of cultured cells (cell density and viability, cell metabolic activity and lactate dehydrogenase activity), the potential of PLLA + PECit nonwovens for application in tissue engineering was established.
以柠檬酸作为构建单元的生物材料显示出用作血管和皮肤组织替代物的潜力。含有聚（1,8 - 辛二醇柠檬酸酯）聚合物基质的植入物商业化的成功为探索基于其他二醇的聚柠檬酸酯提供了理论依据。改变二醇的脂肪族链长可以采用功能设计策略来控制植入物的机械性能、降解特性和表面能。在本工作中，合成了聚（1,2 - 乙二醇柠檬酸酯）并在静电纺丝过程中用作聚乳酸的添加剂。已确定聚柠檬酸酯的含量对非织造布的性能有很大影响：聚合物质量比相等时形态最佳。对所得非织造布进行了表面亲水性、拉伸强度和热性能表征。在其表面进行了L929细胞培养。发现这些材料无细胞毒性且孔隙率适合细胞定植。基于评估培养细胞状况的最重要参数（细胞密度和活力、细胞代谢活性和乳酸脱氢酶活性），确定了PLLA + PECit非织造布在组织工程中的应用潜力。
