Laboratory of Enzymology, Department of Cell Biology, University of Brasília, Brasilia, DF, 70910-900, Brazil.
Faculty of Pharmacy, Laboratory of Pharmaceutical Technology, Lab. FarmaTec., Federal University of Goiás, 74690310, Goiânia, GO, Brazil.
Antonie Van Leeuwenhoek. 2024 Apr 2;117(1):64. doi: 10.1007/s10482-024-01958-w.
Trichoderma harzianum is a filamentous fungus that can act as a mycoparasite, saprophyte, or a plant symbiotic. It is widely used as a biological control agent against phytopathogenic fungi and can also be used for plant growth promotion and biofortification. Interaction between T. harzianum and phytopathogenic fungi involves mycoparasitism, competition, and antibiosis. Extracellular vesicles (EVs) have been described as presenting a central role in mechanisms of communication and interaction among fungus and their hosts. In this study, we characterized extracellular vesicles of T. harzianum produced during growth in the presence of glucose or S. sclerotiorum mycelia. A set of vesicular proteins was identified using proteomic approach, mainly presenting predicted signal peptides.
哈茨木霉是一种丝状真菌，可作为真菌寄生菌、腐生菌或植物共生菌。它被广泛用作防治植物病原菌真菌的生物防治剂，也可用于促进植物生长和生物强化。哈茨木霉与植物病原菌真菌之间的相互作用涉及真菌寄生、竞争和抗生作用。细胞外囊泡（EVs）已被描述为在真菌与其宿主之间的通讯和相互作用机制中发挥核心作用。在这项研究中，我们对在葡萄糖或 Sclerotinia sclerotiorum 菌丝存在下生长的哈茨木霉产生的细胞外囊泡进行了表征。使用蛋白质组学方法鉴定了一组囊泡蛋白，主要呈现预测的信号肽。
