Department of Biochemistry and Immunology, Ribeirao Preto Medical School, University of São Paulo, Ribeirao Preto, São Paulo, Brazil.
Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
mBio. 2022 Feb 22;13(1):e0327221. doi: 10.1128/mbio.03272-21. Epub 2022 Jan 11.
Fungal infections are associated with high mortality rates in humans. The risk of fungal diseases creates the urgent need to broaden the knowledge base regarding their pathophysiology. In this sense, the role of extracellular vesicles (EVs) has been described to convey biological information and participate in the fungus-host interaction process. We hypothesized that fungal EVs work as an additional element in the communication routes regulating fungal responses in intraspecies interaction systems. In this respect, the aim of this study was to address the gene regulation profiles prompted by fungal EVs in intraspecies recipient cells. Our data demonstrated the intraspecies uptake of EVs in pathogenic fungi, such as Candida albicans, Aspergillus fumigatus, and Paracoccidioides brasiliensis, and the effects triggered by EVs in fungal cells. In C. albicans, we evaluated the involvement of EVs in the yeast-to-hypha transition, while in and A. fumigatus the function of EVs as stress transducers was investigated. and A. fumigatus were exposed to an inhibitor of glycosylation or UV light, respectively. The results demonstrated the role of EVs in regulating the expression of target genes and triggering phenotypic changes. The EVs treatment induced cellular proliferation and boosted the yeast to hyphal transition in C. albicans, while they enhanced stress responsiveness in A. fumigatus and , establishing a role for EVs in fungal intraspecies communication. Thus, EVs regulate fungal behavior, acting as potent message effectors, and understanding their effects and mechanism(s) of action could be exploited in antifungal therapies. Here, we report a study about extracellular vesicles (EVs) as communication mediators in fungi. Our results demonstrated the role of EVs from Candida albicans, Aspergillus fumigatus, and Paracoccidioides brasiliensis regulating the expression of target genes and phenotypic features. We asked whether fungal EVs play a role as message effectors. We show that fungal EVs are involved in fungal interaction systems as potent message effectors, and understanding their effects and mechanisms of action could be exploited in antifungal therapies.
真菌感染与人类的高死亡率有关。真菌病的风险使得人们迫切需要扩大对其病理生理学的了解。在这方面，已经描述了细胞外囊泡 (EVs) 在传递生物信息和参与真菌-宿主相互作用过程中的作用。我们假设真菌 EVs 作为调节种内相互作用系统中真菌反应的通信途径的一个额外元素发挥作用。在这方面，本研究的目的是解决真菌 EVs 在种内受体内引发的基因调控谱。我们的数据表明，致病性真菌（如白色念珠菌、烟曲霉和巴西副球孢子菌）能够摄取 EVs，并且 EVs 会在真菌细胞中引发效应。在白色念珠菌中，我们评估了 EVs 在酵母-菌丝过渡中的作用，而在 和烟曲霉中，我们研究了 EVs 作为应激传感器的功能。 和烟曲霉分别暴露于糖基化抑制剂或紫外线照射下。结果表明 EVs 在调节靶基因表达和触发表型变化中的作用。EVs 处理诱导细胞增殖并促进白色念珠菌中的酵母-菌丝过渡，同时增强烟曲霉和 的应激反应，从而确立了 EVs 在真菌种内通信中的作用。因此，EVs 调节真菌行为，充当有效的信息效应物，了解其作用及其作用机制可用于抗真菌治疗。 在这里，我们报告了一项关于细胞外囊泡 (EVs) 作为真菌通信介质的研究。我们的结果表明，来自白色念珠菌、烟曲霉和巴西副球孢子菌的 EVs 调节靶基因表达和表型特征。我们想知道真菌 EVs 是否作为信息效应物发挥作用。我们表明，真菌 EVs 作为有效的信息效应物参与真菌相互作用系统，了解其作用及其作用机制可用于抗真菌治疗。
