Büchner T, Hiddemann W, Wörmann B, Kleinemeier B, Schumann J, Göhde W, Ritter J, Müller K M, von Bassewitz D B, Roessner A
Pathol Res Pract. 1985 Jan;179(3):310-7. doi: 10.1016/s0344-0338(85)80140-0.
The differential pattern of DNA-aneuploidy, detected by flow cytometry (FCM) regarding its frequency, grade and multiclonality, was investigated and correlated to tumor type, malignancy grade, tumor stage and prognosis in a multi-institutional study at the University of Münster. High resolution measurements using admixed normal blood reference cells were undertaken in 2413 cases of 13 different malignant diseases and in 776 benign lesions or samples. The incidence of DNA-aneuploidy was highest in melanomas, carcinomas, testicular tumors, sarcomas (75%-95%) and myelomas (65%). Acute leukemias showed an intermediate DNA-aneuploidy rate of 40% with special subgroups represented by common ALL (44%), p less than 0.05) and myelomonocytic/monocytic AML (47%, p less than 0.01). The lowest DNA-aneuploidy-rate was found in basal cell skin carcinomas (19%) and congenital melanocytic nevi (9%). No case of DNA-aneuploidy was observed in the 776 benign lesions or samples.--DNA-indices giving the grade of DNA-aneuploidy with 1.0 for normal diploid G1/0 cells were found distributed predominantly between 1.0 and 2.0 in the solid tumors, except testicular tumors, clustering around a triploid maximum at 1.5. DNA-indices of myelomas and acute leukemias generally ranged below 1.25 with lower DNA-aneuploidy grades in AML than in ALL (p less than 0.01).--In melanomas the aneuploidy rate was higher (86%) in metastases than in the primary tumors (54%, p = 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)
在明斯特大学开展的一项多机构研究中，对通过流式细胞术（FCM）检测到的DNA非整倍体的差异模式（涉及其频率、分级和多克隆性）进行了研究，并将其与肿瘤类型、恶性程度、肿瘤分期及预后相关联。在13种不同恶性疾病的2413例病例以及776例良性病变或样本中，使用混合正常血液参考细胞进行了高分辨率测量。DNA非整倍体的发生率在黑色素瘤、癌、睾丸肿瘤、肉瘤（75%-95%）和骨髓瘤（65%）中最高。急性白血病的DNA非整倍体率为40%，处于中等水平，常见急性淋巴细胞白血病（44%，p<0.05）和骨髓单核细胞/单核细胞急性髓细胞白血病（47%，p<0.01）等特殊亚组表现出该率。DNA非整倍体率最低的是基底细胞皮肤癌（19%）和先天性黑素细胞痣（9%）。在776例良性病变或样本中未观察到DNA非整倍体病例。——给出DNA非整倍体分级的DNA指数，正常二倍体G1/0细胞为1.0，在实体瘤中（睾丸肿瘤除外）主要分布在1.0至2.0之间，以1.5的三倍体最大值为聚集点。骨髓瘤和急性白血病的DNA指数一般低于1.25，急性髓细胞白血病的DNA非整倍体分级低于急性淋巴细胞白血病（p<0.01）。——在黑色素瘤中，转移灶的非整倍体率（86%）高于原发性肿瘤（54%，p=0.001）。（摘要截选至250词）
