Camphausen R T, Jones R L, Brennan P J
Proc Natl Acad Sci U S A. 1985 May;82(10):3068-72. doi: 10.1073/pnas.82.10.3068.
Mycobacterium paratuberculosis (National Animal Disease Center strain 18 and American Type Culture Collection strain 19698), the causative agent of Johne Disease (bovine paratuberculosis), contains a major immunoreactive glycopeptidolipid (polar GPL-I) that has been isolated and characterized. (formula; see text) Thus, the glycolipid antigen belongs to the polar mycoside C glycopeptidolipid family present in other mycobacterial species. The distal 2,3-di-O-methyl-alpha-L-fucopyranosyl-(1----3)-alpha-L-rhamnopyranosi de unit, the obvious antigen determinant, appears to be characteristic of M. paratuberculosis. The glycolipid can be recognized readily in isolates of M. paratuberculosis by TLC and its presence may be used as a characteristic marker of the infectious agent. The polar glycopeptidolipid was highly reactive in ELISA against serum from an animal hyperimmunized with M. paratuberculosis strain 18, indicating its basic immunogenicity.
副结核分枝杆菌（国家动物疾病中心菌株18和美国典型培养物保藏中心菌株19698）是约内氏病（牛副结核病）的病原体，它含有一种主要的免疫反应性糖肽脂（极性GPL-I），该糖肽脂已被分离并鉴定。（化学式；见正文）因此，这种糖脂抗原属于其他分枝杆菌物种中存在的极性霉菌糖苷C糖肽脂家族。末端的2,3-二-O-甲基-α-L-呋喃岩藻糖基-(1→3)-α-L-鼠李吡喃糖苷单元是明显的抗原决定簇，似乎是副结核分枝杆菌的特征。通过薄层层析法可以很容易地在副结核分枝杆菌分离物中识别出这种糖脂，其存在可作为该感染因子的特征性标志物。这种极性糖肽脂在酶联免疫吸附测定中对用副结核分枝杆菌菌株18进行过超免疫的动物血清具有高度反应性，表明其基本免疫原性。
