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PFGE 诊断和核苷酸测序方法在烹饪员工食物中毒病原体分析中的有用性和局限性。

Usefulness and Limitations of PFGE Diagnosis and Nucleotide Sequencing Method in the Analysis of Food Poisoning Pathogens Found in Cooking Employees.

机构信息

Graduate School of Public Health & Welfare, Konyang University, 158 Gwanjeodong-ro, Daejeon 35365, Republic of Korea.

Chungcheongnam-do Institute of Health and Environment Research, 8 Hongyegongwon-ro, Hongseong 32254, Republic of Korea.

出版信息

Int J Mol Sci. 2024 Apr 8;25(7):4123. doi: 10.3390/ijms25074123.

DOI:10.3390/ijms25074123
PMID:38612932
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11012705/
Abstract

In the case of a food poisoning outbreak, it is essential to understand the relationship between cooking workers and food poisoning. Many biological diagnostic methods have recently been developed to detect food poisoning pathogens. Among these diagnostic tools, this study presents PCR-based pulsed-field gel electrophoresis and nucleotide sequencing diagnostic analysis results for diagnosing food poisoning outbreaks associated with cooking employees in Chungcheongnam-do, Republic of Korea. Pulsed-field gel electrophoresis was useful in identifying the food poisoning outbreaks caused by and In the case of Norovirus, nucleotide sequencing was used to identify the relationship between cooking workers and the food poisoning outbreak. However, it is difficult to determine whether cooking employees directly caused the food poisoning outbreaks based on these molecular biological diagnostic results alone. A system is needed to integrate epidemiological and diagnostic information to identify a direct correlation between the food poisoning outbreak and cooking employees.

摘要

在食物中毒爆发的情况下，了解厨师与食物中毒之间的关系至关重要。最近已经开发出许多生物诊断方法来检测食物中毒病原体。在这些诊断工具中，本研究介绍了基于 PCR 的脉冲场凝胶电泳和核苷酸测序诊断分析结果，用于诊断与韩国忠清南道厨师相关的食物中毒爆发。脉冲场凝胶电泳可用于鉴定 和 引起的食物中毒爆发。在诺如病毒的情况下，使用核苷酸测序来确定厨师与食物中毒爆发之间的关系。然而，仅根据这些分子生物学诊断结果，很难确定厨师员工是否直接导致了食物中毒爆发。需要建立一个系统来整合流行病学和诊断信息，以确定食物中毒爆发与厨师员工之间的直接相关性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ec/11012705/0a3902fe1277/ijms-25-04123-g005a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ec/11012705/32cf52df80ff/ijms-25-04123-g001a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ec/11012705/4abb28978a39/ijms-25-04123-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ec/11012705/e55e69ab579f/ijms-25-04123-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ec/11012705/1501ac818705/ijms-25-04123-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ec/11012705/0a3902fe1277/ijms-25-04123-g005a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ec/11012705/32cf52df80ff/ijms-25-04123-g001a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ec/11012705/4abb28978a39/ijms-25-04123-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ec/11012705/e55e69ab579f/ijms-25-04123-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ec/11012705/1501ac818705/ijms-25-04123-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ec/11012705/0a3902fe1277/ijms-25-04123-g005a.jpg

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