Wen Zhanbo, Yang Wenhui, Li Na, Wang Jie, Hu Lingfei, Li Jinsong, Yin Zhe, Zhang Ke, Dong Xiaokai
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Particuology. 2014 Apr;13:82-87. doi: 10.1016/j.partic.2012.11.009. Epub 2013 Jun 15.
To assess the risk of infectious bacterial aerosols leaking to the environment, the filtration efficiency of a biosafety level 3 (BSL-3) laboratory high-efficiency particulate (HEPA) filter was investigated using the aerosolized bacteria . The aerosol size was measured using an Andersen sampler. Eight first stage HEPA filters (numbered 1-8) were distributed in contaminated labs and exhausts from each of the first stage HEPA filters were aggregated and filtered through one second stage HEPA filter before being released to the environment. In total, 8 first-stage and 1 second-stage HEPA filters from the BSL-3 air purification system were analyzed. No was detected in first stage filters 1, 2, 4, 5, 7 and 8 and the second stage HEPA filter. The filtration efficiencies against aerosolized were >99.9999%. First stage filter numbers 3 and 6 had filtration efficiencies of 99.9825% and 99.9906%, respectively. When filter number 3 was replaced by a new filter and the bracket for filter number 6 was sealed, no aerosolized was detected in the filtered air. Our work suggests that the BSL-3 laboratory HEPA filter air purification system is effective against bacterial aerosols, with little to no bacterial leakage into the environment.
为评估感染性细菌气溶胶泄漏到环境中的风险，使用雾化细菌对生物安全3级（BSL-3）实验室高效微粒（HEPA）过滤器的过滤效率进行了研究。使用安德森采样器测量气溶胶大小。八个一级HEPA过滤器（编号1-8）分布在受污染实验室中，每个一级HEPA过滤器的排气汇总后，在排放到环境之前通过一个二级HEPA过滤器进行过滤。总共对BSL-3空气净化系统的8个一级和1个二级HEPA过滤器进行了分析。在一级过滤器1、2、4、5、7和8以及二级HEPA过滤器中均未检测到[细菌名称未给出]。对雾化[细菌名称未给出]的过滤效率>99.9999%。一级过滤器3和6的过滤效率分别为99.9825%和99.9906%。当过滤器3更换为新过滤器且过滤器6的支架密封后，在过滤空气中未检测到雾化[细菌名称未给出]。我们的工作表明，BSL-3实验室HEPA过滤器空气净化系统对细菌气溶胶有效，几乎没有细菌泄漏到环境中。
