Emam Soha Alaa, Mahran Abeer Hashem, Elshafei Marwa Mokbel
Department of Endodontic, Faculty of Dentistry, Misr International University, Cairo, Egypt.
Department of Endodontic, Faculty of Dentistry, Ain Shams University, Cairo, Egypt.
J Conserv Dent Endod. 2024 Mar;27(3):326-330. doi: 10.4103/JCDE.JCDE_40_24. Epub 2024 Mar 6.
Cytotoxicity and adaptability are among the highly imperative tests that should be performed on a novel endodontic material to ensure its successful implementation in endodontic treatment.
Assess a recently introduced bioceramic root canal sealer CeramoSeal with TotalFill BC and AH plus sealers regarding the cytotoxicity and adaptability.
Five sealer discs were prepared for each sealer and their extracts were cultured in 96-well plates containing human fibroblasts for 24 h. After their incubation, MTT solution was added to each well plate using an enzyme-linked immunosorbent assay plate reader was implemented to calculate the percentage of viable cells. Thirty mandibular single-rooted premolars were prepared using the Edge Endo rotary system, teeth were divided into three groups ( = 10) based on the sealer type: Group 1 CeramoSeal, Group 2 Totalfill, and Group 3 AH plus sealer. Teeth were sectioned longitudinally and viewed under a scanning electron microscope where the region with the gaps was identified and quantified as a percentage of the root canal's overall area.
One-way ANOVA test was used for cytotoxicity, while Kruskal-Wallis and Friedman's tests were used for adaptability.
Ceramoseal statistically significantly showed the lowest viability, at high concentrations AH plus showed the highest cell viability, while at lower concentration Totalfill BC sealer showed the highest cell viability percentage. The gap percentages were statistically significantly higher in Ceramoseal group, there was no statistically significant difference between AH Plus and Totalfill groups.
Ceramoseal sealer exhibited the lowest viability and highest gap percentage compared to the other sealers.
细胞毒性和适应性是对新型牙髓材料进行的极为重要的测试，以确保其在牙髓治疗中成功应用。
评估一种最近推出的生物陶瓷根管封闭剂CeramoSeal与TotalFill BC和AH plus封闭剂在细胞毒性和适应性方面的表现。
为每种封闭剂制备五个封闭剂圆盘，并将其提取物在含有人类成纤维细胞的96孔板中培养24小时。培养后，使用酶联免疫吸附测定酶标仪向每个孔板中加入MTT溶液，以计算活细胞的百分比。使用Edge Endo旋转系统制备30颗下颌单根前磨牙，根据封闭剂类型将牙齿分为三组（每组n = 10）：第1组为CeramoSeal，第2组为Totalfill，第3组为AH plus封闭剂。将牙齿纵向切片，在扫描电子显微镜下观察，确定有间隙的区域，并将其量化为根管总面积的百分比。
单向方差分析用于细胞毒性测试，而Kruskal-Wallis和Friedman检验用于适应性测试。
在统计学上，Ceramoseal在高浓度下显示出最低的细胞活力，AH plus在高浓度下显示出最高的细胞活力，而在低浓度下Totalfill BC封闭剂显示出最高的细胞活力百分比。Ceramoseal组的间隙百分比在统计学上显著更高，AH Plus组和Totalfill组之间没有统计学上的显著差异。
与其他封闭剂相比，Ceramoseal封闭剂表现出最低的细胞活力和最高的间隙百分比。
