Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
Present address: Darwin Bioprospecting, Parc Científic de la Universitat de València c/Catedrático Agustín Escardino 9, 46980 Paterna (Valencia), Spain.
Int J Syst Evol Microbiol. 2020 Sep;70(9):4966-4977. doi: 10.1099/ijsem.0.004367. Epub 2020 Aug 7.
The family comprises prosthecate bacteria with a dimorphic cell cycle and also non-prosthecate bacteria. Cells of all described species divide by binary fission. Strain 0127_4 was isolated from forest soil in Baden Württemberg (Germany) and determined to be the first representative of the family which divided by budding. Cells of strain 0127_4 were Gram-negative, rod-shaped, prosthecate, motile by means of a polar flagellum, non-spore-forming and non-capsulated. The strain formed small white colonies and grew aerobically and chemo-organotrophically utilizing organic acids, amino acids and proteinaceous substrates. 16S rRNA gene sequence analysis indicated that this bacterium was related to TH1-2 and DRW22-8 with 91.3 and 89.7% sequence similarity, respectively. Four unidentified glycolipids were detected as the major polar lipids and, unlike all described members of the family , phosphatidylglycerol was absent. The major fatty acids were summed feature 8 (Cω7/Cω6), summed feature 9 (iso-Cω9/C 10-methyl), C and summed feature 3 (C 6/C 7). The major respiratory quinone was Q-10. The G+C content of the genomic DNA was 63.5 %. Based on the present taxonomic characterization, strain 0127_4 represents a novel species of a new genus, gen. nov., sp. nov. The type strain of is 0127_4 (=DSM 104635=CECT 9243).
该家族包括具二相细胞周期的菌毛变形菌和非菌毛变形菌。所有描述的物种的细胞通过二分分裂进行分裂。0127_4 株从德国巴登符腾堡州的森林土壤中分离得到，被确定为第一个通过出芽方式分裂的家族代表。0127_4 株细胞呈革兰氏阴性、杆状、菌毛变形、通过极生鞭毛运动、不形成孢子、不形成荚膜。该菌株形成小的白色菌落，好氧生长，化能有机营养，利用有机酸、氨基酸和蛋白质底物。16S rRNA 基因序列分析表明，该细菌与 TH1-2 和 DRW22-8 具有 91.3%和 89.7%的序列相似性。检测到四种未鉴定的糖脂作为主要极性脂，与该家族所有描述的成员不同，不存在磷脂酰甘油。主要脂肪酸为总和特征 8（Cω7/Cω6）、总和特征 9（iso-Cω9/C 10-甲基）、C 和总和特征 3（C 6/C 7）。主要呼吸醌为 Q-10。基因组 DNA 的 G+C 含量为 63.5%。基于目前的分类特征，0127_4 株代表了一个新属的新种，属。nov.，种。nov.。的模式菌株为 0127_4（=DSM 104635=CECT 9243）。
