Department of Pathology, Bengbu Medical University, Bengbu, China.
Medicine (Baltimore). 2024 Apr 19;103(16):e37820. doi: 10.1097/MD.0000000000037820.
Aldehyde dehydrogenase 2 (ALDH2) plays a critical role in safeguarding cells against acetaldehyde toxicity and is closely linked to human metabolism. Nevertheless, the involvement of ALDH2 in cancer remains enigmatic. This investigation seeks to comprehensively assess ALDH2's significance in pan-cancer. We conducted an all-encompassing analysis of pan-cancer utilizing multiple databases, including TCGA, linkedomicshs, UALCAN, and Kaplan-Meier plotter. We employed diverse algorithms such as EPIC, MCPCOUNTER, TIDTIMER, xCell, MCP-counter, CIBERSORT, quanTIseq, and EPIC to examine the connection between ALDH2 expression and immune cell infiltration. Single-cell sequencing analysis furnished insights into ALDH2's functional status in pan-cancer. Immunohistochemical staining was performed to validate ALDH2 expression in cancer tissues. In a comprehensive assessment, we observed that tumor tissues demonstrated diminished ALDH2 expression levels compared to normal tissues across 16 different cancer types. ALDH2 expression exhibited a significant positive correlation with the infiltration of immune cells, including CD4 + T cells, CD8 + T cells, neutrophils, B cells, and macrophages, in various tumor types. Moreover, this study explored the association between ALDH2 and patient survival, examined the methylation patterns of ALDH2 in normal and primary tumor tissues, and delved into genetic variations and mutations of ALDH2 in tumors. The findings suggest that ALDH2 could serve as a valuable prognostic biomarker in pan-cancer, closely linked to the tumor's immune microenvironment.
乙醛脱氢酶 2（ALDH2）在保护细胞免受乙醛毒性方面起着至关重要的作用，并且与人类新陈代谢密切相关。然而，ALDH2 在癌症中的作用仍然是一个谜。本研究旨在全面评估 ALDH2 在泛癌症中的意义。我们使用包括 TCGA、linkedomicshs、UALCAN 和 Kaplan-Meier plotter 在内的多个数据库对泛癌症进行了全面分析。我们使用了多种算法，如 EPIC、MCPCOUNTER、TIDTIMER、xCell、MCP-counter、CIBERSORT、quanTIseq 和 EPIC，来研究 ALDH2 表达与免疫细胞浸润之间的关系。单细胞测序分析提供了泛癌症中 ALDH2 功能状态的见解。我们进行了免疫组织化学染色来验证癌症组织中 ALDH2 的表达。在全面评估中，我们观察到 16 种不同癌症类型的肿瘤组织中 ALDH2 的表达水平低于正常组织。ALDH2 表达与包括 CD4+T 细胞、CD8+T 细胞、中性粒细胞、B 细胞和巨噬细胞在内的免疫细胞在各种肿瘤类型中的浸润呈显著正相关。此外，本研究还探讨了 ALDH2 与患者生存的关系，检查了正常和原发性肿瘤组织中 ALDH2 的甲基化模式，并深入研究了肿瘤中 ALDH2 的遗传变异和突变。研究结果表明，ALDH2 可以作为泛癌症中一种有价值的预后生物标志物，与肿瘤的免疫微环境密切相关。
