College of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi 830011, Xinjiang, China.
College of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi 830011, Xinjiang, China.
Ultrason Sonochem. 2021 Dec;80:105833. doi: 10.1016/j.ultsonch.2021.105833. Epub 2021 Nov 15.
This study was designed to optimize the extraction rate of total polyphenols and ellagic acid from pomegranate flowers. Single factors were investigated for liquid-to-material ratio (5-25), ethanol concentration (20%-60%), sonication time (5-60 min), and sonication power (150-500 W). The level range of the Box-Bokhen design was determined with respect to the single-factor results. The components of each index were normalized using the entropy weighting method for obtaining the comprehensive evaluation value. Under the actual conditions, the final optimization results were 17 for liquid-to-material ratio, 43% for ethanol concentration, 10 min for ultrasonic time, and 300 W for ultrasonic power. The extracts obtained under optimal conditions were tested for the inhibition of Streptococcus mutans and its biofilm, and results showed that pomegranate flowers exerted some inhibitory effects on the bacterium. Phosphomolybdenum and FRAP assays were used, and DPPH, ABTS, and O radical scavenging tests were conducted, indicating that pomegranate flower extracts have good antioxidant capacity.
本研究旨在优化从石榴花中提取总多酚和鞣花酸的提取率。考察了液料比（5-25）、乙醇浓度（20%-60%）、超声时间（5-60min）和超声功率（150-500W）等单因素对提取效果的影响。根据单因素实验结果，确定了 Box-Bokhen 设计的水平范围。采用熵权法对各指标成分进行归一化处理，得到综合评价值。在实际条件下，最终优化结果为液料比 17、乙醇浓度 43%、超声时间 10min、超声功率 300W。在最佳条件下提取的提取物对变形链球菌及其生物膜的抑制作用进行了测试，结果表明石榴花对该细菌有一定的抑制作用。采用磷钼蓝法和 FRAP 法进行检测，并进行 DPPH、ABTS 和 O 自由基清除试验，表明石榴花提取物具有良好的抗氧化能力。
