一种模块化3D打印微流控系统：大规模生物加工中连续细胞收获的潜在解决方案。
A modular 3D printed microfluidic system: a potential solution for continuous cell harvesting in large-scale bioprocessing.
作者信息
Ding Lin, Razavi Bazaz Sajad, Asadniaye Fardjahromi Mahsa, McKinnirey Flyn, Saputro Brian, Banerjee Balarka, Vesey Graham, Ebrahimi Warkiani Majid
机构信息
School of Biomedical Engineering, University of Technology Sydney, Sydney, NSW, 2007, Australia.
School of Engineering, Macquarie University, Sydney, NSW, 2109, Australia.
出版信息
Bioresour Bioprocess. 2022 Jun 6;9(1):64. doi: 10.1186/s40643-022-00550-2.
Microfluidic devices have shown promising applications in the bioprocessing industry. However, the lack of modularity and high cost of testing and error limit their implementation in the industry. Advances in 3D printing technologies have facilitated the conversion of microfluidic devices from research output to applicable industrial systems. Here, for the first time, we presented a 3D printed modular microfluidic system consisting of two micromixers, one spiral microfluidic separator, and one microfluidic concentrator. We showed that this system can detach and separate mesenchymal stem cells (MSCs) from microcarriers (MCs) in a short time while maintaining the cell's viability and functionality. The system can be multiplexed and scaled up to process large volumes of the industry. Importantly, this system is a closed system with no human intervention and is promising for current good manufacturing practices.
微流控装置在生物加工行业已展现出具有前景的应用。然而，缺乏模块化以及测试成本高和误差限制了它们在该行业的应用。3D打印技术的进步促进了微流控装置从研究成果向适用的工业系统的转化。在此，我们首次展示了一种3D打印的模块化微流控系统，该系统由两个微混合器、一个螺旋微流控分离器和一个微流控浓缩器组成。我们表明，该系统能够在短时间内从微载体（MCs）上分离间充质干细胞（MSCs），同时保持细胞的活力和功能。该系统可以进行多路复用并扩大规模以处理大量工业生产。重要的是，该系统是一个无需人工干预的封闭系统，有望符合现行良好生产规范。
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