Fuhren Jori, Schwalbe Markus, Boekhorst Jos, Rösch Christiane, Schols Henk A, Kleerebezem Michiel
Host Microbe Interactomics Group, Wageningen University & Research, Wageningen, The Netherlands.
Laboratory of Food Chemistry, Wageningen University & Research, Wageningen, The Netherlands.
Gut Microbes. 2024 Jan-Dec;16(1):2338946. doi: 10.1080/19490976.2024.2338946. Epub 2024 Apr 24.
Synbiotics combine the concepts of probiotics and prebiotics to synergistically enhance the health-associated effects of both components. Previously, we have shown that the intestinal persistence of inulin-utilizing Lp900 is significantly increased in rats fed an inulin-supplemented, high-calcium diet. Here we employed a competitive population dynamics approach to demonstrate that inulin and GOS can selectively enrich strains that utilize these substrates for growth during cultivation, but that such enrichment did not occur during intestinal transit in rats fed a GOS or inulin-supplemented diet. The intestinal persistence of all strains increased irrespective of their prebiotic utilization phenotype, which was dependent on the calcium level of the diet. Analysis of fecal microbiota and intestinal persistence decline rates indicated that prebiotic utilization capacity did not selectively stimulate intestinal persistence in prebiotic supplemented diets. Moreover, microbiota and organic acid profile analyses indicate that the prebiotic utilizing probiotic strains are vastly outcompeted by the endogenous prebiotic-utilizing microbiota, and that the collective enhanced persistence of all strains is most likely explained by their well-established tolerance to organic acids.
合生元结合了益生菌和益生元的概念，以协同增强两种成分对健康的相关影响。此前，我们已经表明，在喂食补充菊粉的高钙饮食的大鼠中，利用菊粉的Lp900在肠道中的持久性显著增加。在这里，我们采用了一种竞争性种群动力学方法来证明，菊粉和低聚半乳糖（GOS）可以在培养过程中选择性地富集利用这些底物生长的菌株，但在喂食补充GOS或菊粉饮食的大鼠的肠道转运过程中不会发生这种富集。所有菌株在肠道中的持久性都增加了，无论它们的益生元利用表型如何，这取决于饮食中的钙水平。对粪便微生物群和肠道持久性下降率的分析表明，益生元利用能力在补充益生元的饮食中不会选择性地刺激肠道持久性。此外，微生物群和有机酸谱分析表明，利用益生元的益生菌菌株在很大程度上被内源性利用益生元的微生物群所竞争，所有菌株共同增强的持久性最有可能是由它们对有机酸的良好耐受性来解释的。
