Host Microbe Interactomics Group, Wageningen University and Research, Wageningen, the Netherlands.
Food Chemistry Group, Wageningen University and Research, Wageningen, the Netherlands.
Appl Environ Microbiol. 2020 Sep 1;86(18). doi: 10.1128/AEM.01081-20.
Synbiotics are food supplements that combine probiotics and prebiotics to synergistically elicit a health effect in humans. exhibits remarkable genetic and phenotypic diversity, in particular in strain-specific carbohydrate utilization capacities, and several strains are marketed as probiotics. We have screened 77 strains for their abilities to utilize specific prebiotic fibers, revealing variable and strain-specific growth efficiencies on isomalto- and galactooligosaccharides. We identified a single strain within the screening panel that was able to effectively utilize inulin and fructooligosaccharides (FOS), which did not support efficient growth of the rest of the strains. In the panel we tested, we did not find strains that could utilize arabinoxylooligosaccharides or sulfated fucoidan. The strain-specific growth phenotype on isomaltooligosaccharides was further analyzed using high-performance anion-exchange chromatography, which revealed distinct substrate utilization phenotypes within the strain panel. The strain-specific phenotypes could be linked to the strains' genotypes by identifying gene clusters coding for carbohydrate membrane transport systems that are predicted to be involved in the utilization of isomaltose and other (unidentified) oligosaccharides in the isomaltooligosaccharide substrate. Synbiotics combine prebiotics and probiotics to synergistically enhance the health benefits associated with these ingredients. is encountered as a natural inhabitant of the gastrointestinal tract, and specific strains are marketed as probiotics based on their strain-specific health-promoting activities. Strain-specific stimulation of growth through prebiotic substrates could enhance the persistence and/or activity of Our study establishes a high-throughput screening model for prebiotic substrate utilization by individual strains of bacteria, which can be readily employed for synbiotic matchmaking approaches that aim to enhance the intestinal delivery of probiotics through strain-specific, selective growth stimulation.
合生素是将益生菌和益生元结合起来，协同发挥对人类健康有益作用的食品补充剂。 具有显著的遗传和表型多样性，特别是在菌株特异性碳水化合物利用能力方面，有几种菌株被作为益生菌销售。我们筛选了 77 株菌，以检测它们利用特定益生元纤维的能力，结果显示在异麦芽低聚糖和半乳糖低聚糖上的生长效率存在可变性和菌株特异性。我们在筛选面板中鉴定出一株能够有效利用菊粉和低聚果糖（FOS）的单一菌株，而其余菌株则不能有效地支持其生长。在所测试的面板中，我们没有发现能够利用阿拉伯木聚糖低聚糖或硫酸化褐藻聚糖的菌株。使用高效阴离子交换色谱法进一步分析异麦芽低聚糖上的菌株特异性生长表型，结果揭示了菌株组内不同的底物利用表型。通过鉴定编码碳水化合物膜转运系统的基因簇，可以将菌株特异性生长表型与菌株的基因型联系起来，这些基因簇被预测参与异麦芽糖和异麦芽低聚糖底物中其他（未鉴定）寡糖的利用。合生素将益生元和益生菌结合起来，协同增强这些成分与健康相关的益处。 是胃肠道的天然寄居菌，特定菌株因其菌株特异性的促进健康的活性而被作为益生菌销售。通过益生元底物刺激菌株特异性生长可以增强 的持久性和/或活性。我们的研究建立了一种高通量筛选模型，用于检测单个细菌菌株对益生元底物的利用，该模型可以用于合生素匹配方法，旨在通过菌株特异性、选择性生长刺激来增强益生菌在肠道中的传递。
