Bian Shuanling, Zhao Mengliang, Zhang Huijuan, Ren Yanjing
State Key Laboratory of Plateau Ecology and Agriculture, Laboratory of Research and Utilization of Germplasm Resources in Qinghai-Tibet Plateau, Qinghai University, Xining 810016, China.
Key Laboratory of Germplasm Resources Protection and Genetic Improvement of the Qinghai-Tibet Plateau in Ministry of Agriculture and Rural, Xining 810016, China.
Plants (Basel). 2024 Apr 22;13(8):1167. doi: 10.3390/plants13081167.
Osmotic stress is a condition in which plants do not get enough water due to changes in environmental factors. Plant response to osmotic stress is a complex process involving the interaction of different stress-sensitive mechanisms. Differentially expressed genes and response mechanisms of kohlrabi have not been reported under osmotic stress. A total of 196,642 unigenes and 33,040 differentially expressed unigenes were identified in kohlrabi seedlings under polyethylene glycol osmotic stress. /, and eight other transcription factor family members with a high degree of interaction with CAT and SOD antioxidant enzyme activity were identified. Subsequently, 151 / genes were identified and analyzed. Twelve conserved motifs were searched and all / genes were clustered into four groups. A total of 149 / genes were randomly distributed on the chromosome, and relative expression level analysis showed that / genes of kohlrabi have obvious specificity in different tissues. This study lays a foundation for explaining the osmotic stress resistance mechanism of kohlrabi and provides a theoretical basis for the functional analysis of / transcription factor family members.
渗透胁迫是一种由于环境因素变化导致植物无法获得足够水分的状况。植物对渗透胁迫的响应是一个复杂的过程，涉及不同胁迫敏感机制的相互作用。在渗透胁迫下，尚未有关于甘蓝型油菜差异表达基因和响应机制的报道。在聚乙二醇渗透胁迫下，甘蓝型油菜幼苗中共鉴定出196,642个单基因和33,040个差异表达单基因。/, 并鉴定出与CAT和SOD抗氧化酶活性具有高度相互作用的其他八个转录因子家族成员。随后，鉴定并分析了151个/基因。搜索到十二个保守基序，所有/基因被聚类为四组。共有149个/基因随机分布在染色体上，相对表达水平分析表明，甘蓝型油菜的/基因在不同组织中具有明显的特异性。本研究为解释甘蓝型油菜的抗渗透胁迫机制奠定了基础，并为/转录因子家族成员的功能分析提供了理论依据。
