Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, China.
Department of Laboratory Medicine, Affliated Hospital 2 of Nantong University, Nantong, China.
Technol Cancer Res Treat. 2024 Jan-Dec;23:15330338241249032. doi: 10.1177/15330338241249032.
Receve revealed that hemoglobin beta (HBB) plays an important role not only in blood disorders but also in malignancies. The aim of this study is to investigate the clinical significance, diagnostic value, and biological function of HBB in lung cancer. HBB expression was examined in lung cancer tissues and plasma samples using quantitative real-time polymerase chain reaction, and its relationship with clinical pathological characteristics was analyzed. Receiver operating characteristic (ROC) curves were constructed to evaluate the diagnostic value of HBB in lung cancer. The proliferation of A549 and SPCA1 cells was analyzed using a cell counting kit-8 assay and protein expressions were detected by western blot. The expressions of HBB were found to be down-regulated in both lung cancer tissues and plasma samples. Notably, plasma HBB levels were significantly elevated in postoperative samples when compared to their preoperative counterparts. Across 66 cases of lung cancer tissues, a correlation was observed between HBB levels and both gender and tumor, node, metastasis staging. ROC curve analysis further confirmed the high diagnostic potential of HBB expression in lung cancer. Moreover, the combination of HBB and carcinoembryonic antigen (CEA) had greater significance than HBB or CEA alone in the diagnosis of lung cancer. Knocking out or overexpressing HBB could affect lung cancer cell proliferation through the ERK1/2 signaling pathway. HBB can serve as a novel biomarker for the diagnosis and monitoring of lung cancer, regulating cell proliferation via the ERK1/2 pathway and playing a pivotal role in the oncogenesis and progression of the disease.
瑞切维揭示了血红蛋白β（HBB）不仅在血液疾病中，而且在恶性肿瘤中都发挥着重要作用。本研究旨在探讨 HBB 在肺癌中的临床意义、诊断价值和生物学功能。采用实时定量聚合酶链反应检测肺癌组织和血浆样本中的 HBB 表达，并分析其与临床病理特征的关系。构建Receiver operating characteristic（ROC）曲线评估 HBB 在肺癌中的诊断价值。使用细胞计数试剂盒-8 分析 A549 和 SPCA1 细胞的增殖，并通过 Western blot 检测蛋白表达。结果发现，HBB 在肺癌组织和血浆样本中的表达均下调。值得注意的是，与术前相比，术后样本中的血浆 HBB 水平显著升高。在 66 例肺癌组织中，HBB 水平与性别和肿瘤、淋巴结、转移分期均存在相关性。ROC 曲线分析进一步证实了 HBB 表达在肺癌诊断中的高潜力。此外，HBB 与癌胚抗原（CEA）的联合检测比 HBB 或 CEA 单独检测在肺癌诊断中的意义更大。敲除或过表达 HBB 可通过 ERK1/2 信号通路影响肺癌细胞增殖。HBB 可作为肺癌诊断和监测的新型生物标志物，通过 ERK1/2 通路调节细胞增殖，在肿瘤发生和进展中发挥关键作用。
