Richardson H J, Elford P R, Sharrard R M, Meats J E, Russell R G
Cell Immunol. 1985 Jan;90(1):41-51. doi: 10.1016/0008-8749(85)90167-4.
We have investigated the relationship between the monokine interleukin 1 (IL-1) and the connective tissue-stimulating activities produced by monocytes such as mononuclear cell factor (MCF). Using almost exclusively human tissue we have monitored a wide range of MCF-like activities through the partial purification of IL-1 by gel filtration and isoelectric focusing. Activities measured include stimulation of chondrocytes to produce prostaglandins, plasminogen activator and proteoglycanase, enhancement of synovial cell proliferation, and stimulation of cartilage resorption, in addition to IL-1 (lymphocyte activating factor) activity. The activities described show the same molecular heterogeneity; the active material has similar potencies in the different systems, and removal of IL-1 activity by pretreatment with phenylglyoxal also results in loss of the connective tissue-stimulating activities. These results show that the factors responsible for this wide range of activities are very closely related to IL-1 and give further evidence in support of the possible involvement of IL-1 in the processes of joint destruction occurring in chronic inflammatory conditions such as rheumatoid arthritis.
我们研究了单核因子白细胞介素1（IL-1）与单核细胞产生的结缔组织刺激活性之间的关系，如单核细胞因子（MCF）。几乎仅使用人体组织，我们通过凝胶过滤和等电聚焦对IL-1进行部分纯化，监测了广泛的MCF样活性。所测量的活性包括刺激软骨细胞产生前列腺素、纤溶酶原激活剂和蛋白聚糖酶，增强滑膜细胞增殖，刺激软骨吸收，以及IL-1（淋巴细胞激活因子）活性。所描述的活性表现出相同的分子异质性；活性物质在不同系统中具有相似的效力，用苯乙二醛预处理去除IL-1活性也会导致结缔组织刺激活性丧失。这些结果表明，负责这种广泛活性的因子与IL-1密切相关，并进一步证明IL-1可能参与类风湿关节炎等慢性炎症性疾病中发生的关节破坏过程。
