School of Life Sciences, Nantong University, Nantong, 226019, Jiangsu, China.
Key Laboratory of Landscape Plant Genetics and Breeding, Nantong University, NO.9 Seyuan Road, Nantong, 226019, Jiangsu, China.
Genetica. 2024 Jun;152(2-3):101-117. doi: 10.1007/s10709-024-00208-1. Epub 2024 May 10.
DnaJs/Hsp40s/JPDs are obligate co-chaperones of heat shock proteins (Hsp70), performing crucial biological functions within organisms. A comparative genome analysis of four genomes (Vitis vinifera, Eucalyptus grandis, Lagerstroemia indica, and Punica granatum) revealed that the DnaJ gene family in L. indica has undergone expansion, although not to the extent observed in P. granatum. Inter-genome collinearity analysis of four plants indicates that members belonging to Class A and B are more conserved during evolution. In L. indica, the expanded members primarily belong to Class-C. Tissue expression patterns and the biochemical characterization of LiDnaJs further suggested that DnaJs may be involved in numerous biological processes in L. indica. Transcriptome and qPCR analyses of salt stressed leaves identified at least ten LiDnaJs that responded to salt stress. In summary, we have elucidated the expansion mechanism of the LiDnaJs, which is attributed to a recent whole-genome triplication. This research laid the foundation for functional analysis of LiDnaJs and provides gene resources for breeding salt-tolerant varieties of L. indica.
DnaJs/Hsp40s/JPDs 是热休克蛋白（Hsp70）的必需共伴侣，在生物体中发挥着至关重要的生物学功能。对四个基因组（葡萄、桉树、紫薇和石榴）的比较基因组分析表明，紫薇中的 DnaJ 基因家族发生了扩张，但程度不及石榴。四个植物的种间共线性分析表明，A 类和 B 类成员在进化过程中更为保守。在紫薇中，扩张的成员主要属于 C 类。LiDnaJs 的组织表达模式和生化特性进一步表明，DnaJs 可能参与了紫薇的许多生物学过程。对盐胁迫叶片的转录组和 qPCR 分析鉴定出至少十个对盐胁迫有响应的 LiDnaJs。总之，我们阐明了 LiDnaJs 的扩张机制，这归因于最近的全基因组三倍体化。这项研究为 LiDnaJs 的功能分析奠定了基础，并为培育紫薇耐盐品种提供了基因资源。
