Simmons J G, Fuller C R, Buchanan P D, Yount W J
Scand J Immunol. 1981 Jul;14(1):1-13. doi: 10.1111/j.1365-3083.1981.tb00178.x.
Twenty-two IgG-positive human lymphoblastoid cell lines and normal peripheral blood lymphocytes were studied for surface and cytoplasmic IgG, IgG subclasses, IgD and IgM, using monospecific fluorescein- and rhodamine-conjugated F(ab')2 antibody fragments, and for secretion by double antibody radioimmunoassay. Several parallel observations and several differences in IgG subclass expression were noted between cell lines and normal lymphocytes. Surface IgG2 was frequently expressed in normal IgG-positive lymphocytes but was seldom expressed in cell lines. Cell lines resembled normal IgG-positive lymphocytes in the frequent expression of cytoplasmic IgG3 and IgG4, often without secretion. Cell lines and normal lymphocytes both showed more frequent distribution of IgG and IgG subclasses in cytoplasm than in surface immunoglobulin, and often a discrepancy of surface versus cytoplasmic IgG subclass. A good correlation was noted between surface, cytoplasmic and secreted IgG1. Despite a predominance of IgG2 and IgG4 surface IgG subclasses, and IgG3 and IgG1 in cytoplasm, secreted immunoglobulins from normal lymphocytes in short-term culture showed a similar distribution of IgG subclasses to that seen in normal sera. Multiple expression of IgG subclasses was much more frequent in IgG-positive cell lines than in normal peripheral blood lymphocytes, both in surface and cytoplasmic IgG.
使用单特异性荧光素和罗丹明偶联的F(ab')2抗体片段，对22株IgG阳性的人淋巴母细胞系和正常外周血淋巴细胞进行了表面和细胞质IgG、IgG亚类、IgD和IgM的研究，并通过双抗体放射免疫测定法检测其分泌情况。在细胞系和正常淋巴细胞之间观察到了一些平行现象以及IgG亚类表达方面的一些差异。表面IgG2在正常IgG阳性淋巴细胞中经常表达，但在细胞系中很少表达。细胞系在细胞质IgG3和IgG4的频繁表达方面类似于正常IgG阳性淋巴细胞，且通常不分泌。细胞系和正常淋巴细胞均显示，IgG及其亚类在细胞质中的分布比表面免疫球蛋白更为频繁，并且表面与细胞质IgG亚类之间常常存在差异。表面、细胞质和分泌型IgG1之间存在良好的相关性。尽管表面IgG亚类以IgG2和IgG4为主，细胞质中以IgG3和IgG1为主，但短期培养的正常淋巴细胞分泌的免疫球蛋白显示出与正常血清中相似的IgG亚类分布。在IgG阳性细胞系中，IgG亚类的多重表达在表面和细胞质IgG中均比正常外周血淋巴细胞更为频繁。
