Akramov A R, Kalinkovich A G, Pinegin B V
Zh Mikrobiol Epidemiol Immunobiol. 1985 Mar(3):82-6.
The injection of 5 X 10(7) sheep red blood cells (SRBC) to CBA and (CBA X C57BL/6)F1 mice was accompanied by the formation of antigen-induced B-suppressors (AIBs) in the spleen of the animals on day 7. When transferred to syngeneic intact recipients, AIBs inhibited their response to SRBC (control). Methotrexate in a dose of 2.5 mg/kg, cytozar in a dose of 1000 mg/kg and 5-fluorouracyl in a dose of 100 mg/kg, injected to donors simultaneously with SRBC (group A), had no influence on the number of IgM-forming cells and, when introduced on day 5 after the injection of SRBC, decreased the number of such cells in donors (group B) as determined by testing the donors' response on day 7. The suppressor activity of AIBs obtained from the donors of group A was equal to that of the control, while AIBs obtained from the donors of group B had lower suppressor activity in comparison with the control. These results suggest that the proliferation of the precursors of AIBs occurs between days 2 and 5 of immune response and the formation of AIBs themselves, between days 5 and 7.
给CBA和（CBA×C57BL/6）F1小鼠注射5×10⁷个绵羊红细胞（SRBC）后，在第7天动物脾脏中会形成抗原诱导的B抑制细胞（AIB）。当将AIB转移至同基因的未受影响受体时，它们会抑制受体对SRBC的反应（对照）。在给供体注射SRBC的同时，注射剂量为2.5mg/kg的甲氨蝶呤、剂量为1000mg/kg的阿糖胞苷和剂量为100mg/kg的5-氟尿嘧啶（A组），对IgM形成细胞的数量没有影响，而在注射SRBC后第5天注射这些药物时，通过检测供体在第7天的反应发现，供体中此类细胞的数量减少（B组）。从A组供体获得的AIB的抑制活性与对照相等，而从B组供体获得的AIB与对照相比具有较低的抑制活性。这些结果表明，AIB前体的增殖发生在免疫反应的第2天至第5天之间，而AIB本身的形成发生在第5天至第7天之间。
