Wagner C R, Vetto R M, Burger D R
Cell Immunol. 1985 Jun;93(1):91-104. doi: 10.1016/0008-8749(85)90391-0.
Activation of T cells requires three signals from an antigen-presenting cell: antigen, Ia determinants (HLA-D region determinants in man), and interleukin 1 (IL-1). Recent evidence has suggested that macrophages, dendritic cells, epidermal Langerhan's cells, and endothelial cells can each function as antigen-presenting cells (APC). If these cell types can independently function as APC, they should synthesize Ia determinants and secrete IL-1. To determine if endothelial cells fulfill these requirements, we have propagated human umbilical vein endothelial cells by serial subculture for extended periods of time and assessed Ia expression and IL-1 secretion. The endothelial cells were subcultured for 8 months (approximately 20 subcultures) and were found to display classic morphology and immunofluorescent staining for the endothelial cell-specific marker Factor VIII-related antigen. In a separate paper we have shown that these subcultured endothelial cells can present antigen to T cells in a HLA-D region-restricted fashion (C. R. Wagner, R. M. Vetto, and D. R. Burger, Subcultured human endothelial cells can independently function as fully competent antigen-presenting cells, accepted for publication, Hum. Immunol.). In this paper we present evidence demonstrating that extensively subcultured endothelial cells biosynthesize both HLA-DR and HLA-DS molecules after exposure to T cells and antigen or to a supernatant from antigen-activated T cells. Evidence is also presented that when endothelial cells are cultured in the presence of lipopolysaccharide they secrete a molecule(s) with IL-1 activity as assayed by LBRM-33-IA5 cell line production of interleukin 2.
T细胞的激活需要来自抗原呈递细胞的三种信号：抗原、Ia决定簇（人类中的HLA - D区域决定簇）和白细胞介素1（IL - 1）。最近的证据表明，巨噬细胞、树突状细胞、表皮朗格汉斯细胞和内皮细胞都可以作为抗原呈递细胞（APC）发挥作用。如果这些细胞类型能够独立作为APC发挥作用，它们应该合成Ia决定簇并分泌IL - 1。为了确定内皮细胞是否满足这些要求，我们通过连续传代培养长时间培养人脐静脉内皮细胞，并评估Ia表达和IL - 1分泌情况。内皮细胞传代培养了8个月（约20次传代），发现其呈现出典型的形态，并对内皮细胞特异性标志物VIII因子相关抗原进行免疫荧光染色。在另一篇论文中我们已经表明，这些传代培养的内皮细胞能够以HLA - D区域限制的方式将抗原呈递给T细胞（C.R.瓦格纳、R.M.韦托和D.R.伯格，传代培养的人内皮细胞可独立作为完全有功能的抗原呈递细胞，已被接受发表，《人类免疫学》）。在本文中，我们提供证据表明，经过广泛传代培养的内皮细胞在接触T细胞和抗原或抗原激活的T细胞的上清液后，会生物合成HLA - DR和HLA - DS分子。还提供了证据表明，当内皮细胞在脂多糖存在的情况下培养时，它们会分泌一种具有IL - 1活性的分子，这是通过LBRM - 33 - IA5细胞系产生白细胞介素2来测定的。
