Opt Lett. 2024 May 15;49(10):2637-2640. doi: 10.1364/OL.519330.
Optical-resolution photoacoustic microscopy (OR-PAM) excels in precisely imaging a biological tissue based on absorption contrast. However, existing OR-PAMs are confined by fixed compromises between spatial resolution and field of view (FOV), preventing the integration of large FOV and local high-resolution within one system. Here, we present a non-telecentric OR-PAM (nTC-PAM) that empowers efficient adaptation of FOV and spatial resolution to match the multi-scale requirement of diverse biological imaging. Our method allows for a large-scale transformation in FOV and even surpassing the nominal FOV of the objective with minimal marginal degradation of the lateral resolution. We demonstrate the advantage of nTC-PAM through multi-scale imaging of the leaf phantom, mouse ear, and cortex. The results reveal that nTC-PAM can switch the FOV and spatial resolution to meet the requirements of different biological tissues, such as large-scale imaging of the whole cerebral cortex and high-resolution imaging of microvascular structures in local brain regions.
光学分辨率光声显微镜（OR-PAM）擅长基于吸收对比精确成像生物组织。然而，现有的 OR-PAM 受到空间分辨率和视场（FOV）之间固定折衷的限制，无法在一个系统中集成大 FOV 和局部高分辨率。在这里，我们提出了一种非远心 OR-PAM（nTC-PAM），它能够有效地调整 FOV 和空间分辨率，以满足不同生物成像的多尺度要求。我们的方法允许 FOV 进行大规模转换，甚至可以超过物镜的名义 FOV，而横向分辨率的边缘退化最小。我们通过叶状模型、鼠耳和皮质的多尺度成像展示了 nTC-PAM 的优势。结果表明，nTC-PAM 可以切换 FOV 和空间分辨率以满足不同生物组织的要求，例如整个大脑皮层的大规模成像和局部脑区微血管结构的高分辨率成像。
