Dagogo-Jack S, Atkinson S, Kendall-Taylor P
J Immunoassay. 1985;6(1-2):125-36. doi: 10.1080/01971528508063025.
Epidermal growth factor (EGF) is a small polypeptide with potent mitogenic activity. Its synthesis by mouse submaxillary gland is stimulated by certain hormones. To assess its physiological significance in man, we have developed a homologous radioimmunoassay for human epidermal growth factor (hEGF) in saliva. A satisfactory standard curve was readily obtained using either buffer or peptide-free saliva. The mean IC50 was 436 +/- 200 pM (mean +/- SD) and sensitivity approximately 35 pM. The mean normal salivary hEGF in 63 males was 314.6 +/- 21.7 pM (+/- SEM) and 354 +/- 27.8 pM in 48 females. The difference between the means of the sexes was not significant. Assays of aliquots stored under different conditions showed hEGF in saliva to be stable and the method reproducible. Salivary hEGF secretion did not suggest diurnal rhythmicity and was unrelated to meals.
表皮生长因子（EGF）是一种具有强大促有丝分裂活性的小多肽。小鼠颌下腺对其合成受某些激素刺激。为评估其在人类中的生理意义，我们开发了一种用于检测唾液中人类表皮生长因子（hEGF）的同源放射免疫测定法。使用缓冲液或无肽唾液均可轻松获得满意的标准曲线。平均半数抑制浓度（IC50）为436±200皮摩尔（平均±标准差），灵敏度约为35皮摩尔。63名男性唾液中hEGF的平均正常水平为314.6±21.7皮摩尔（±标准误），48名女性为354±27.8皮摩尔。两性均值之间的差异不显著。对在不同条件下储存的等分试样进行的检测表明，唾液中的hEGF稳定，且该方法可重复。唾液hEGF分泌未显示出昼夜节律性，且与进餐无关。
